Results 81 to 90 of about 950,178 (298)

2015 Limited access state numbered highways.

open access: yes, 2014
Annual; Each v. reports data for the previous year; e.g. 1987 v. presents 1986 data.; Issued by: Road Inventory and Records Unit, ; Engineering Data and Inventory Section, -1982; Division of Planning Inventory and Data, 1983-1990; Planning Inventory and ...

core   +1 more source

Advancing carbon estimation in harvested wood products in the United States: a case study in the Northern Lake States

open access: yesCarbon Balance and Management
Background Carbon stocks and stock changes in harvested wood products (HWPs) are an important part of land sector greenhouse gas (GHG) estimation and reporting.
Joseph M. Nash, Grant M. Domke
doaj   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

open access: yesFEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang   +10 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Site classification vs. wood production: a case study based on Silver fir growth dynamics in the Western Carpathians

open access: yesJournal of Forest Science, 2011
We analysed wood production on an example of Silver fir growth ability within site units defined in the site classification that is currently used in the Western Carpathians.
M. Bošeľa, R. Petráš, Š. Šmelko
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Pinyon-juniper specialist birds are resilient to local-scale reduction of canopy cover and pinyon pines, but amount of understory shrub cover determines the composition of the insectivore guild

open access: yesAvian Conservation and Ecology
Drought-induced tree die-off and die-back in the southwestern United States is changing the structure and floristic composition of pinyon-juniper woodlands, a widespread middle-elevation community with high bird endemism.
Harrison H Jones   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Use of UAV Photogrammetric Data for Estimation of Biophysical Properties in Forest Stands Under Regeneration

open access: yesRemote Sensing, 2019
The objective of this study was to assess the use of unmanned aerial vehicle (UAV) data for modelling tree density and canopy height in young boreal forests stands.
Stefano Puliti   +2 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

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