Results 81 to 90 of about 694,399 (289)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

The Application of Remote Sensing Technology in Inland Water Quality Monitoring and Water Environment Science: Recent Progress and Perspectives

Remote Sensing
Due to its long-term and high-frequency observation capabilities, remote sensing is widely recognized as an indispensable and preferred technology for large-scale and cross-regional water quality monitoring.
Lei Chen, Leizhen Liu, Shasha Liu, Zhenyu Shi, Chunhong Shi   +4 more
doaj   +1 more source

Underwater Topography Inversion in Liaodong Shoal Based on GRU Deep Learning Model

Remote Sensing, 2020
The Liaodong Shoal in the east of the Bohai Sea has obvious water depth variation. The clear shallow water area and deep turbid area coexist, which is characterized by complex submarine topography.
Zihao Leng, Jie Zhang, Yi Ma, Jingyu Zhang   +3 more
doaj   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

Identification and detection of high NO x emitting inland ships using multi-source shore-based monitoring data

Environmental Research Letters
In urban areas situated along busy waterways like the Yangtze River, the diesel engines of inland navigation ships emerge as significant contributors to air pollution. Among these vessels, certain high-emission ships exhibit considerably higher levels of
Hongxun Huang, Chunhui Zhou, Changshi Xiao, Yuanqiao Wen, Weihao Ma, Lichuan Wu   +5 more
doaj   +1 more source

Research on the Inversion Method of Dust Retention in Grassland Plant Canopies Based on UAV-Borne Hyperspectral Data

Land
Monitoring the dust retention content in grassland plants around open-pit coal mines is of significant importance for environmental pollution monitoring and the development of dust control strategies.
Yibo Zhao, Shaogang Lei
doaj   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

The response mechanism and testing method of the rock elastic modulus while drilling

Geomatics, Natural Hazards & Risk
The elastic modulus is a basic parameter reflecting the deformation resistance and energy concentration characteristics of rock. The traditional testing method of the rock elastic modulus needs to core the surrounding rock and testing it in the ...
Bei Jiang, Fenglin Ma, Hongke Gao, Qi Wang, Songlin Cai, Chong Zhang, Zhenguo Bian, Guangjie Liu   +7 more
doaj   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source