Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography. [PDF]
J Chromatogr A, 2015 Protein separation is an integral step in biopharmaceutical manufacture with diffusion-limited packed bed chromatography remaining the default choice for industry.
Dods SR +3 more
europepmc +5 more sources
Ion-exchange chromatography purification of extracellular vesicles
BioTechniques, 2017 Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović +4 more
doaj +2 more sources
Total plasma homocysteine measurement: Evaluation of the Abbott immunoassay, comparison with the JEOL ion exchange chromatography and investigation of its clinical utility [PDF]
Practical Laboratory Medicine, 2022 Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen +6 more
doaj +2 more sources
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline. [PDF]
J Vis Exp, 2017 Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules.
Brennich ME, Round AR, Hutin S.
europepmc +2 more sources
Online ion-exchange chromatography for small-angle X-ray scattering. [PDF]
Acta Crystallogr D Struct Biol, 2016 SAXS coupled with online ion-exchange chromatography allows the collection of high-quality BioSAXS data.
Hutin S, Brennich M, Maillot B, Round A.
europepmc +2 more sources
Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]
BiomedicinesBackground: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
doaj +2 more sources
Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration.
Jelena Ruscic +4 more
doaj +2 more sources
Antibody Aggregate Removal by Multimodal Chromatography [PDF]
MoleculesThe growing demand for therapeutic monoclonal antibodies (mAbs) has heightened the need for efficient and scalable purification strategies. A major challenge in downstream processing is the removal of antibody aggregates, which can compromise drug safety,
Veronika Rupčíková +3 more
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PURIFICATION AND PARTIAL CHARACTERIZATION OF SERUM IMMUNOGLOBULIN FROM PERSIAN STURGEON (ACIPENSER PERSICUS) [PDF]
Majallah-i ̒Ilmī-i Shīlāt-i Īrān, 2003 In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel ...
M. Kalbasi1; A. Mostafaei2; J. Majidi3
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Ion Exchange Chromatography - An Overview [PDF]
, 2013 Morsi, Rania E., Moustafa, Yasser M.
core +5 more sources