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Analytical Chemistry, 2015
Characterization of biopharmaceutical products is a challenging task, which needs to be carried out at several different levels (including both primary structure and conformation).
Khaja Muneeruddin +2 more
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Characterization of biopharmaceutical products is a challenging task, which needs to be carried out at several different levels (including both primary structure and conformation).
Khaja Muneeruddin +2 more
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Analytical Chemistry, 2017
Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes.
Bhumit A. Patel +12 more
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Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes.
Bhumit A. Patel +12 more
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Modeling of salt and pH gradient elution in ion-exchange chromatography.
Journal of Separation Science, 2014The separation of proteins by internally and externally generated pH gradients in chromatofocusing on ion-exchange columns is a well-established analytical method with a large number of applications.
Michael Schmidt +2 more
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[3] Ion-exchange chromatography
1996Publisher Summary Over the years, electrophoretic techniques were the leading bioanalytical tools for biopolymers. Slab gel electrophoresis has yielded spectacular results, in resolving closely related nucleic acids of high molecular mass. Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) have served well ...
Gargi Choudhary, Csaba Horváth
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, 2015
C-phycocyanin, a natural blue dye, stands out today because it has antioxidant, anti-inflammatory, and anti-tumor properties, and it can be extracted from cyanobacteria, such as the genus Spirulina. A process that has received attention is the adsorption
C. C. Moraes +5 more
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C-phycocyanin, a natural blue dye, stands out today because it has antioxidant, anti-inflammatory, and anti-tumor properties, and it can be extracted from cyanobacteria, such as the genus Spirulina. A process that has received attention is the adsorption
C. C. Moraes +5 more
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Analytica Chimica Acta, 1967
Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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Chapter 22 Ion-Exchange Chromatography
2009Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois Jungbauer, Rainer Hahn
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THE PURIFICATION OF TRYPSINOGEN BY ION-EXCHANGE CHROMATOGRAPHY
Canadian Journal of Biochemistry and Physiology, 1962The purification of trypsinogen by several methods of ion-exchange chromatography has been investigated. The use of phosphate–urea buffers on Bio-Rex 70 was not a suitable preparative procedure because of considerable inactivation of the zymogen. Chromatography on carboxymethyl cellulose at pH 3.2 produced no significant increase in the potential ...
G. Y. Mabbott +2 more
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Journal of Separation Science, 2013
Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers
Kecheng Li +6 more
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Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers
Kecheng Li +6 more
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Robust mechanistic modeling of protein ion-exchange chromatography.
Journal of Chromatography A, 2021Vijesh Kumar +6 more
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