Results 51 to 60 of about 1,306,526 (282)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Ion track formation via electric-field-enhanced energy deposition

Physical Review Research
High-energy ion irradiation deposits extreme energy in a narrow range (∼1–10 nm) along ion trajectories in solid through electronic energy loss, producing unique irradiation effects such as ion tracks.
Zikang Ge, Jinhao Hu, Shengyuan Peng, Wei Kang, Xiaofei Shen, Yanbo Xie, Jianming Xue   +6 more
doaj   +1 more source

Nanostructures evolution assessment and spectroscopic properties modification induced by electronic energy loss in KTaO3 crystal

Materials & Design, 2022
Modifications of micro/nanostructure and photoelectric properties under swift ion irradiation (645 MeV Xe35+) of KTaO3 crystal with varying electronic energy losses (7.2–31.4 keV/nm) and ion velocities (0.18–5.00 MeV/u) have been studied by combining ...
Xinqing Han, Eva Zarkadoula, Qing Huang, Miguel L. Crespillo, Cong Liu, Meng Zhang, Xuelin Wang, Peng Liu   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Ions leaving no tracks

The paths of swift heavy ions are typically traceable in solids, because of confined electronic interactions along the paths, inducing what is known in literature as 'ion tracks', i.e. nano-sized in cross-section cylindrical zones of modified material extending for microns in length.
Abdullaev, Azat, Fernandez, Javier Garcia, Nozais, Chloe, O'Connell, Jacques, Tlegenov, Rustem, Sekerbayev, Kairolla, Azarov, Alexander, Leino, Aleksi, Bouvier, Tomas Fernandez, Zhao, Junlei, Pena, Aldo Artimez, Medvedev, Nikita, Utegulov, Zhandos, Prytz, Oystein, Djurabekova, Flyura, Kuznetsov, Andrej   +15 more
openaire   +2 more sources

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

High-Energy ion tracks in thin films

, 2006
High-energy ion tracks (374 MeV Au(26+)) in thin films were examined with transmission electron microscopy to investigate nanopore formation. Tracks in quartz and mica showed diffraction contrast.
DOYLE B. L., ROSSI, PAOLO, NORMAN A. K., BRINGA E. M., MCDANIEL F. D., FOLLSTAEDT D. M.   +5 more
core   +1 more source

Lithium-Ion Battery Management System: A Lifecycle Evaluation Model for the Use in the Development of Electric Vehicles

MATEC Web of Conferences, 2018
The use of Lithium-ion batteries in the automobile sector has expanded drastically in the recent years. The foreseen increment of lithium to power electric and hybrid electric vehicles has provoked specialists to analyze the long term credibility of ...
Sisodia Ayush, Monteiro Jonathan
doaj   +2 more sources

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source