Results 111 to 120 of about 3,526,732 (300)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Compatibility of pronounced detachment with improved confinement on HL-2A tokamak

Nuclear Fusion
This paper investigates the compatibility of pronounced detachment with improved confinement based on the NBI-heated HL-2A L-mode plasma with low-density.
Ting Wu, Min Xu, Zhuo Wang, Lin Nie, Zhanhui Wang, Jinming Gao, Yihang Chen, Yiren Zhu, Yi Zhang, Liang Liu, Dong Li, Kai Zhang, Rui Ke, Xiaoxue He, Zengceng Yang, Xin Yu, Na Wu, Zhihui Huang, Kaiyang Yi, Weice Wang, Longwen Yan, Yonggao Li, Ting Long, Renxu Wang, Wenjing Tian, Zhe Wang, Laizhong Cai, Yi Yu   +27 more
doaj   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Determining Partitioning Rate Constants and Diffusion Coefficients in Ion-exchange Membranes Using Time-dependent Partitioning Measurements

Electrochemistry
This study investigates ion partitioning and transport mechanisms within ion-exchange membranes (IEMs) and their influence on electrodialysis (ED) permselectivity.
Yu SUGIMOTO
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Speeding up adiabatic ion transport in macroscopic multi-Penning-trap stacks for high-precision experiments

Communications Physics
Multi-Penning traps are an excellent tool for high-precision tests of fundamental physics in a variety of applications, ranging from atomic mass measurements to symmetry tests.
Moritz von Boehn, Jan Schaper, Julia A. Coenders, Johannes Brombacher, Teresa Meiners, Malte Niemann, Juan M. Cornejo, Stefan Ulmer, Christian Ospelkaus   +8 more
doaj   +1 more source

Swedish urban transport model powered by biomethane [PDF]

, 2019
In the recent years, European Community has been implementing a plan to increase the share of renewable fuels used for energy production and powering vehicles.
Żółtowski, Andrzej; Motor Transport Institute
core  

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source