Results 91 to 100 of about 390,019 (267)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

MXene membrane with directionally functionalized channel entrances for enhanced ion selectivity and permeability

Nature Communications
Ion-separation membranes that combine high selectivity with high permeability are important for various practical applications. Molecular functionalization of sub-nanochannel membranes is widely used to improve ion separation selectivity; however, it ...
Hang Yu, Rongming Xu, Jiachun Ren, Ling Yuan, Zheng Cui, Weiming Zhang, Zhuyuan Wang, Lu Lv, Xiwang Zhang, Bingcai Pan   +9 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Environmental concentrations of N-nitrosodiethylamine (NDEA) disturb the Ca2 + and K+ homeostasis in the gills and epidermis of mosquitofish (Gambusia affinis)

Ecotoxicology and Environmental Safety
N-nitrosodiethylamine (NDEA), a nitrogenous disinfection by-product, is notorious for its ubiquitous presence in the environment and its carcinogenic properties. However, its impact on ion homeostasis in aquatic organisms remains underexplored.
Xiaoyu Yan, Wenwen Chen, Xiaohong Song, Yun Ma, Haiqin Wang, Tao Yang, Yanpeng Liang, Honghu Zeng   +7 more
doaj   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Characterization of Lithium Ion Transport Via Dialysis Process [PDF]

Iranian Journal of Chemical Engineering, 2006
Dialysis is a membrane based separation process in which the concentration gradient across the membrane is the driving force resulting in a flow of material from one side of the membrane to the other. In this study membranes (Accurel, Celgard, GVHP, PM30
doaj  

Integrating First Principles Calculations and Machine Learning to Study the ReNiO2/Ti3C2 Heterojunctions for Sodium Ion Batteries

Advanced Physics Research
Due to the large size of sodium ions and their slow redox kinetics in electrochemical processes, the sodium ion batteries currently are still far from satisfactory.
Yuanyuan Cui, Chengyu Zhang, Luxin Niu, Jiao Zheng, Xin Liu, Sihan Yang, Yanfeng Gao   +6 more
doaj   +1 more source