Results 51 to 60 of about 2,134,301 (294)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Ionic strength dependence of polyelectrolyte brush thickness [PDF]
Soft Matter, 2012 Intramolecular repulsion between charged monomers imposes intrinsic tension in polyion, modifies its elasticity, and changes the dependence of polyelectrolyte brush thickness on salt concentration. We demonstrate that intrinsic polyion tension gives rise to a plateau of the brush thickness in the middle of salt dominated regime and to a subsequent ...
Ekaterina B, Zhulina +1 more
openaire +2 more sources
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Effects of humic acid and ionic strength on TiO2 nanoparticles sublethal toxicity to zebrafish
, 2015 The stability and bioavailability of titanium dioxide nanoparticles (TiO2 NPs) suspension could be modified by the physicochemical properties of solution.
Fang, T. +3 more
core +1 more source
Electrostatic stretching of grafted maleic acid copolymer chains
eXPRESS Polymer Letters, 2009 We report on the swelling behaviour of thin maleic acid copolymer films in dependence of pH and ionic strength. The response of the polymer films was studied by quartz crystal microbalance with dissipation monitoring.
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
, 2014 Citation: 'ionic strength' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.I03180 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire +1 more source
Prospecting the protein design landscape
FEBS Letters, EarlyView.This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona +4 more
wiley +1 more source
A Numerical Study of Chemical Compatibility of GCLs
Applied Sciences, 2022 A series of COMSOL numerical models were established to study the chemical compatibility of GCLs (geosynthetic clay liner). The effect of chemistry on the mesoscopic structure and the hydraulic conductivity of GCLs was investigated.
Juan Hou +4 more
doaj +1 more source