The influence of the polar head-group of synthetic cationic lipids on the transfection efficiency mediated by niosomes in rat retina and brain [PDF]
, 2016 The development of novel non-viral delivery vehicles is essential in the search of more efficient strategies for retina and brain diseases. Herein, optimized niosome formulations prepared by oil-in water (o/w) and film-hydration techniques were ...
Agirre, Mireia +11 more
core +1 more source
Communications Biology, 2021 Carrasco et al. report a method that can predict the lipid nanoparticles (LNP) pKa from the structure of the ionizable lipid. They investigate the delivery efficiency for intramuscular and intravascular administration and propose design principles to ...
Manuel J. Carrasco +10 more
doaj +1 more source
Niosomes based on synthetic cationic lipids for gene delivery: The influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells [PDF]
, 2015 We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes ...
Agirre, Mireia +10 more
core +1 more source
Ionizable Amphiphilic Dendrimer-Based Nanomaterials with Alkyl-Chain-Substituted Amines for Tunable siRNA Delivery to the Liver Endothelium In Vivo [PDF]
, 2014 A library of dendrimers was synthesized and optimized for targeted small interfering RNA (siRNA) delivery to different cell subpopulations within the liver.
Akinc +46 more
core +1 more source
Charge regulation: a generalized boundary condition?
, 2016 The three most commonly-used boundary conditions for charged colloidal systems are constant charge (insulator), constant potential (conducting electrode) and charge regulation (ionizable groups at the surface).
Andelman, David +2 more
core +1 more source
Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis [PDF]
, 2006 Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins.
Chevallet, Mireille +3 more
core +4 more sources
Electrospray ionization mass spectrometry of lysoglycerophosphocholine lipid subclasses.
Journal of the American Society for Mass Spectrometry, 2000 Lysoglycerophosphocholine lipids (lyso-GPC) are important intermediates in the synthesis and metabolism of glycerophosphocholine lipids which are major components of the cellular lipid bilayer. Significant differences in the collisional induced decomposition (CID) behavior were observed for each of the four different subtypes of lyso-GPC in both ...
Khaselev, Nona, Murphy, Robert C
openaire +2 more sources
Mass spectrometric imaging of lipids using desorption electrospray ionization [PDF]
Journal of Chromatography B, 2009 Desorption electrospray ionization (DESI), a relatively new ambient ionization technique used in mass spectrometry (MS), allows for the direct analysis of samples such as thin tissue sections, to be conducted outside of vacuum in the ambient environment and often without sample preparation.
Allison L, Dill +4 more
openaire +2 more sources
Ionizable lipid nanoparticles with functionalized PEG-lipids increase retention in the tumor microenvironment [PDF]
Mol Ther Methods Clin DevThis study explores the efficacy of ionizable lipid nanoparticles (LNPs) modified with various functionalized polyethylene glycol (PEG)-lipids for retention within the tumor microenvironment after intratumoral (IT) injection. LNPs were synthesized and characterized with four different functionalized PEG-lipids, and the top performing lipids were ...
Jester M +6 more
europepmc +3 more sources
Salt-induced reentrant stability of polyion-decorated particles with tunable surface charge density
, 2015 The electrostatic complexation between DOTAP-DOPC unilamellar liposomes and an oppositely charged polyelectrolyte (NaPA) has been investigated in a wide range of the liposome surface charge density.
Bordi, Federico +3 more
core +3 more sources