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Characterization of genetic diversity of animal and human Mycobacterium avium strains by IS1245-IS1311 spacer typing [PDF]
A PCR method previously developed for typing Mycobacterium avium was used to characterize the genetic diversity of M. avium strains isolated from swine (n = 90) and humans (n = 24). The strains were identified with IS901 PCR and IS1245 PCR: 38 strains were of IS901+ and IS1245+ genotype (M. avium subsp.
M. Pate +3 more
exaly +4 more sources
IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil [PDF]
Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and ...
Panunto, A.C. +2 more
exaly +7 more sources
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains [PDF]
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All
Patrícia Carvalho de Sequeira +3 more
exaly +6 more sources
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Diagnostic Microbiology and Infectious Disease, 2021
Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan.
Thoko Flav Kapalamula +2 more
exaly +3 more sources
Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan.
Thoko Flav Kapalamula +2 more
exaly +3 more sources
IS1245 genotypic analysis of Mycobacterium avium isolates from patients in Brazil
Disseminated Mycobacterium avium infection is an emerging opportunistic disease among patients with acquired immunodeficiency syndrome (AIDS) in Brazil. The mode of transmission of M. avium in a developing country setting needs to be better characterized.Mycobacterium avium strain collections in São Paulo and Rio de Janeiro were analyzed according to ...
Moises Palaci, Afranio L Kritski
exaly +3 more sources
Scandinavian Journal of Infectious Diseases, 2005
Cervical lymphadenitis is the main manifestation of Mycobacterium avium infection in immunocompetent children. Exposure to birds has been discussed as a source of infection. To clarify from where children acquire the infection, M. avium isolates from different origins were analysed with restriction fragment length polymorphism (RFLP) on insertion ...
Johanna Thegerström
exaly +3 more sources
Cervical lymphadenitis is the main manifestation of Mycobacterium avium infection in immunocompetent children. Exposure to birds has been discussed as a source of infection. To clarify from where children acquire the infection, M. avium isolates from different origins were analysed with restriction fragment length polymorphism (RFLP) on insertion ...
Johanna Thegerström
exaly +3 more sources
Evaluation of IS1245-based PCR for Detection of Mycobacterium avium Bacteraemia in AIDS Patients
Scandinavian Journal of Infectious Diseases, 1999A PCR method based on the repetitive IS1245 sequence was evaluated for the detection of Mycobacterium avium bacteraemia in AIDS patients. Two blood preparation methods were applied: lysis of erythrocytes using a hypotonic buffer and Ficoll density centrifugation.
A, Roth +4 more
exaly +3 more sources
Comparative Immunology, Microbiology and Infectious Diseases, 2008
Mycobacterium avium subsp. hominissuis, ubiquitous environmental mycobacterium, is an opportunistic pathogen that is regularly isolated from pigs and humans in Slovenia. Genetic diversity of 114 isolates from pigs (n = 57) and humans (n = 57), identified by means of bacteriology, DNA-RNA hybridization techniques, IS901 PCR and IS1245 PCR, was ...
Mateja, Pate +3 more
openaire +2 more sources
Mycobacterium avium subsp. hominissuis, ubiquitous environmental mycobacterium, is an opportunistic pathogen that is regularly isolated from pigs and humans in Slovenia. Genetic diversity of 114 isolates from pigs (n = 57) and humans (n = 57), identified by means of bacteriology, DNA-RNA hybridization techniques, IS901 PCR and IS1245 PCR, was ...
Mateja, Pate +3 more
openaire +2 more sources

