Results 151 to 160 of about 725 (172)

A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness

Journal of Clinical Microbiology, 1995
The insertion sequence IS1245 is a novel mycobacterial repetitive element identified in Mycobacterium avium. It encodes a transposase which exhibits a 64% amino acid similarity with IS1081, an insertion element present in the M. tuberculosis complex. The host range of IS1245 appears limited to M.
C, Guerrero, C, Bernasconi, D, Burki, T, Bodmer, A, Telenti   +4 more
openaire   +2 more sources

Computer-assisted analysis of Mycobacterium avium fingerprints using insertion elements IS1245 and IS1311 in a Caribbean setting

Research in Microbiology, 1997
A total of 33 clinical isolates of the Mycobacterium avium complex from 25 patients, identified by means of biochemical and cultural characteristics, the Accuprobe system and DT1/DT6 PCR, were further analysed using novel insertion elements IS1245 and IS1311 in a French Caribbean setting. PvuII-cleaved DNA and non-radioactive Southern hybridization and
A, Devallois, N, Rastogi
openaire   +2 more sources

High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis

Veterinary Microbiology, 2007
Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the ...
T, Tirkkonen, J, Pakarinen, A-M, Moisander, J, Mäkinen, H, Soini, T, Ali-Vehmas   +5 more
openaire   +2 more sources

IS1245 RFLP analysis of strains of Mycobacterium avium subspecies hominissuis isolated from pigs with tuberculosis lymphadenitis in Portugal

Veterinary Record, 2009
From the end of 2004 increasing numbers of tuberculosis‐like lesions were detected in regular meat inspections of pigs at regional abattoirs. Mycobacterium avium subspecies hominissuis was identified as the cause of this outbreak of tuberculosis ...
M, Domingos, A, Amado, A, Botelho
openaire   +2 more sources

Comparison of IS1245 restriction fragment length polymorphism and pulsed-field gel electrophoresis for typing clinical isolates of Mycobacterium avium subsp avium.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 2000
Little is still known about the epidemiology and pathogenesis of Mycobacterium avium subsp avium (MASA) infection.Examination of the reproducibility and the stability over time of pulsed-field gel electrophoresis (PFGE) and IS1245 restriction fragment length polymorphism (IS1245-RFLP) techniques.
X, Garriga, P, Cortés, P, Rodríguez, F, March, G, Prats, P, Coll   +5 more
openaire   +4 more sources

IS 1311 and IS 1245 Restriction Fragment Length Polymorphism Analyses, Serotypes, and Drug Susceptibilities of Mycobacterium avium Complex Isolates Obtained from a Human Immunodeficiency Virus-Negative Patient

Journal of Clinical Microbiology, 2002
Six isolates of Mycobacterium avium of genotype dnaJ(+) IS901(-) IS1311(+) IS1245(+) and serotypes 6 (n = 1), 6/9, (n = 2), and 9 (n = 3) were obtained within a 5-month period from a human immunodeficiency virus-negative patient treated for tuberculosis. The isolates were identified with PvuII restriction fragment length polymorphism (RFLP) analysis as
Ivo Pavlik
exaly   +2 more sources

Identification of members of Mycobacterium avium species by Accu-Probes, serotyping, and single IS900, IS901, IS1245 and IS901-flanking region PCR with internal standards

Journal of Microbiological Methods, 2006
From Mycobacterium avium species Mycobacterium avium subsp. paratuberculosis (n=961), Mycobacterium a. avium (n=677), Mycobacterium a. silvaticum (n=5), and Mycobacterium a. hominissuis (n=1566) were examined, and from Mycobacterium tuberculosis complex M. tuberculosis (n=2), Mycobacterium bovis (n=13), M.
Milan, Bartos, Pavel, Hlozek, Petra, Svastova, Lenka, Dvorska, Tim, Bull, Ludmila, Matlova, Ilona, Parmova, Isolde, Kuhn, Janine, Stubbs, Monika, Moravkova, Jaromir, Kintr, Vladimir, Beran, Ivan, Melicharek, Matjaz, Ocepek, Ivo, Pavlik   +14 more
openaire   +2 more sources

Distribution of Mycobacterium avium subsp. avium and M. a. hominissuis in artificially infected pigs studied by culture and IS901 and IS1245 quantitative real time PCR

Veterinary Microbiology, 2010
Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) belong to the Mycobacterium avium complex (MAC) and are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included
I, Slana, M, Kaevska, P, Kralik, A, Horvathova, I, Pavlik   +4 more
openaire   +2 more sources

Identification of Mycobacterium avium Genotypes with Distinctive Traits by Combination of IS 1245 -Based Restriction Fragment Length Polymorphism and Restriction Analysis of hsp65

Journal of Clinical Microbiology, 2003
One-hundred eight Mycobacterium avium isolates from pigs, humans, birds, and bovines were typed by the IS1245-based restriction fragment length polymorphism (RFLP) method and PCR-restriction enzyme analysis (PRA) of hsp65. Nine clusters of isolates showing more than 80% similarity in their RFLP profiles were detected.
R S, Oliveira, M P, Sircili, E M D, Oliveira, S C, Balian, J S, Ferreira-Neto, S C, Leão   +5 more
exaly   +2 more sources

Impact of sawdust and wood shavings in bedding on pig tuberculous lesions in lymph nodes, and IS1245 RFLP analysis of Mycobacterium avium subsp. hominissuis of serotypes 6 and 8 isolated from pigs and environment

Veterinary Microbiology, 2004
Among 25,027 slaughter pigs raised in two farms, tuberculous lesions were detected in the lymph nodes of 898 (3.6%) of them. Tuberculous lesions were most commonly found in the mesenteric (601; 2.4%) and head (451; 1.8%) lymph nodes. Mycobacteria were isolated from 49 of 120 randomly selected mesenteric, head and bronchial lymph nodes with diagnosed ...
L, Matlova, L, Dvorska, K, Palecek, L, Maurenc, M, Bartos, I, Pavlik   +5 more
openaire   +2 more sources