Detection of Mycobacterium avium subsp. paratuberculosis in asymptomatic bovines by IS900 Polymerase Chain Reaction [PDF]
Veterinary World, 2011 Faecal samples were collected from 58 asymptomatic bovines and after DNA extraction IS900 Polymerase Chain Reaction (IS900 PCR) was performed to detect Mycobacterium avium subsp. paratuberculosis (MAP). Ten samples (17.24 %) were positive for MAP.
Vijayakumar K +4 more
doaj +4 more sources
Comparitive study on the efficacy of ELISA and IS900 PCR for the diagnosis of paratuberculosis in goats [PDF]
Biotechnology in Animal Husbandry, 2014 Paratuberculosis, one of the chronic granulomatous enteritis that predominantly affects ruminants worldwide, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is most efficiently diagnosed by MAP from faeces by Polymerase Chain Reaction (
Abraham A. +3 more
doaj +4 more sources
Journal of Pure and Applied Microbiology, 2017 Mycobacterium avium subsp. paratuberculosis is a pathogen that causes johne’s disease in animals and is implicated in Crohn’s disease in humans. Culture of Mycobacterium avium subsp.
Sumiyah Rasool +3 more
doaj +3 more sources
HEMATO-BIOCHEMICAL AND MOLECULAR MARKERS (IS900) OF CATTLE INFECTED WITH JOHNE’S DISEASE IN EGYPT [PDF]
Slovenian Veterinary Research, 2019 In Egypt, limited studies were performed to investigate Jhon’s disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP). This study was concerned with diagnosis of MAP in cattle dairy farms using indirect-ELISA technique and ...
Ahmed Abdelaal +7 more
doaj +7 more sources
Journal of Advanced Veterinary and Animal Research, 2014 Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease (JD) in cattle, sheep, goats and other ruminants, and Crohn’s disease in humans. MAPs are shed to external environment through feces and milk.
Bharathy Sukumar +3 more
doaj +3 more sources
Rapid identification of Mycobacterium avium ssp paratuberculosis laboratory strains by IS900-Nested polymerase chain reaction [PDF]
International Journal of Mycobacteriology, 2016 Introdution: Mycobacterium avium ssp paratuberculosis (MAP) causes paratuberculosis (Johne's disease) in ruminants. As a species, M. avium comprises M. avium subsp.
Mohammad Mohammad Taheri +7 more
doaj +4 more sources
Genotyping analysis of bovine, ovine, and caprine paratuberculosis in Iran: An IS900-RFLP study [PDF]
International Journal of Mycobacteriology, 2016 Objective/background: Mycobacterium avium subspecies paratuberculosis (MAP) has been reported in Iranian cattle since 1965. Little is known on the population genetics of MAP in this Middle-Eastern State.
Lida Abdolmohammadi Khiav +3 more
doaj +4 more sources
LCD array and IS900 efficiency in relation to traditional diagnostic techniques for diagnosis of Mycobacterium avium subspecies paratuberculosis in cattle in Egypt [PDF]
International Journal of Mycobacteriology, 2014 This study aimed to compare traditional tests (Johnin test, fecal staining and fecal culture) with advanced laboratory tests (ELISA, LCD array and IS900 PCR) for detection of Johne's disease.
Mohamed Sabry Abd ElRaheam ElSayed
doaj +4 more sources
Identification of Mycobacterium avium subspecies paratuberculosis in raw and pasteurized milk samples using culture, IS900 PCR and IS900 nested PCR methods [PDF]
Bihdāsht-i Mavādd-i Ghaz̠āyī, 2021 Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne’s disease, is a very slow-growing bacterium that imposes heavy costs on livestock-related industries. Due to the similar clinical and pathological symptoms in Johne’s and
M. Soltani
doaj +3 more sources
Effect of IS900 Gene of Mycobacterium paratuberculosis on Mycobacterium smegmatis
Current Microbiology, 1998 Mycobacterium paratuberculosis is mycobactin dependent and contains multiple copies of the IS900 gene that encodes for p43 (46.5K protein). The correlation between the two characteristics has been investigated. A 3.2-kb BamHI fragment from M. paratuberculosis containing the 1.451 kb IS900 gene was cloned in Escherichia coli and Mycobacterium smegmatis ...
S A, Naser +3 more
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