Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy [PDF]
AbstractMycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease or paratuberculosis, a chronic infection affecting domestic ruminants worldwide. Despite sporadic reports of MAP occurrence in non-ruminants, information on the risk factors predisposing for infection is still scarce and evidence of transmission paths ...
Cunha, Mónica V. +6 more
openaire +4 more sources
Identification and strain determination of M. paratuberculosis (MAP) by PCR and REA methods based on IS900 and IS1311 insertion segments [PDF]
The purpose of this study was to identifying the M. avium paratuberculosis (MAP), investigating its outbreaks and optimizing a fast, accurate and simple method based on PCR and RFLP for detection of this subspecies and their strains.
Mohammadreza Nassiri +4 more
doaj +2 more sources
Introduction: Mycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis or Johne’s disease that causes chronic intestinal inflammation in ruminants. The IS900 insertion sequence, specific for Map, hasbeen used widely as an epidemiological marker and target for qPCR diagnosis.
Conde, Cyril +7 more
core +4 more sources
DIAGNOSTIC APPLICATION OF ELISA AND IS900 PCR USING BUFFY COAT AS A SOURCE SAMPLE FOR THE DETECTION OF Mycobacterium avium SUBSPECIES Paratuberculosis IN SUBCLINICAL CASES OF BOVINE PARATUBERCULOSIS [PDF]
John's disease or paratuberculosis is a chronic mycobacterial infection that affectsruminants, adversely, leading to huge economic losses throughout the world.
Kawther K. jaber +2 more
doaj +3 more sources
Detection of
Background Since Mycobacterium avium subspecies paratuberculosis (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not ...
Stephan Roger, Corti Sabrina
doaj +2 more sources
Detection of Mycobacterium avium Sub sp. paratuberculosis in Pasteurized Milk Samples in Northeast of Iran by Culture, Direct Nested PCR and PCR Methods [PDF]
Mycobacterium avium Subsp. paratuberculosis (MAP) is a gram-positive, small, acid-fast bacillus with high environmental resistance. In animals, especially ruminants, it leads to Paratuberculosis (PTB) or Johne's disease, which is chronic granulomatous ...
Nasim Sadeghi +2 more
doaj +1 more source
Efficiency of direct microbial diagnosis, IS900 PCR and microbial culture for detection of Mycobacterium avium subsp. Paratuberculosis in the feces of apparently healthy cattle [PDF]
Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.
Y Anzabi +2 more
doaj +1 more source
Abstract Background Paratuberculosis is a worldwide endemic infectious disease of ruminants that results in high economic losses. Public health concerns are also being raised with human Crohn’s disease. Therefore, control is becoming priority for governments.
Harshita Gandhi +5 more
openaire +2 more sources
Mycobacterium avium subsp. paratuberculosis (MAP) en un hato lechero de búfalos en Colombia
Objetivo. Determinar la presencia de Mycobacterium avium subsp. paratuberculosis (MAP) mediante métodos serológicos, microbiológicos y moleculares, para genotipar los aislamientos de MAP y explorar factores asociados a la seropositividad a MAP en un hato
Brahian Camilo Tuberquia-López +5 more
doaj +1 more source
p43, the protein product of the atypical insertion sequence IS900, is expressed in Mycobacterium paratuberculosis [PDF]
The novel mycobacterial insertion sequence IS900 was analysed by coupled transcription-translation, of both strands independently, in a cell-free E. coli extract using an exogenous promoter. This revealed only one protein product, p43, as predicted from the nucleotide sequence. The protein was readily translated in recombinant E.
M L, Tizard +4 more
openaire +2 more sources

