Results 21 to 30 of about 1,684 (179)

Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy [PDF]

open access: yesScientific Reports, 2020
AbstractMycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease or paratuberculosis, a chronic infection affecting domestic ruminants worldwide. Despite sporadic reports of MAP occurrence in non-ruminants, information on the risk factors predisposing for infection is still scarce and evidence of transmission paths ...
Cunha, Mónica V.   +6 more
openaire   +4 more sources

Identification and strain determination of M. paratuberculosis (MAP) by PCR and REA methods based on IS900 and IS1311 insertion segments [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2012
The purpose of this study was to identifying the M. avium paratuberculosis (MAP), investigating its outbreaks and optimizing a fast, accurate and simple method based on PCR and RFLP for detection of this subspecies and their strains.
Mohammadreza Nassiri   +4 more
doaj   +2 more sources

Whole-Genome Analysis of Mycobacterium avium subsp. paratuberculosis IS900 distribution reveals strain typespecific modalities

open access: yes, 2021
Introduction: Mycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis or Johne’s disease that causes chronic intestinal inflammation in ruminants. The IS900 insertion sequence, specific for Map, hasbeen used widely as an epidemiological marker and target for qPCR diagnosis.
Conde, Cyril   +7 more
core   +4 more sources

DIAGNOSTIC APPLICATION OF ELISA AND IS900 PCR USING BUFFY COAT AS A SOURCE SAMPLE FOR THE DETECTION OF Mycobacterium avium SUBSPECIES Paratuberculosis IN SUBCLINICAL CASES OF BOVINE PARATUBERCULOSIS [PDF]

open access: yesBasrah Journal of Veterinary Research, 2018
John's disease or paratuberculosis is a chronic mycobacterial infection that affectsruminants, adversely, leading to huge economic losses throughout the world.
Kawther K. jaber   +2 more
doaj   +3 more sources

Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland [PDF]

open access: yesBMC Microbiology, 2002
Background Since Mycobacterium avium subspecies paratuberculosis (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not ...
Stephan Roger, Corti Sabrina
doaj   +2 more sources

Detection of Mycobacterium avium Sub sp. paratuberculosis in Pasteurized Milk Samples in Northeast of Iran by Culture, Direct Nested PCR and PCR Methods [PDF]

open access: yesIranian Journal of Chemistry & Chemical Engineering, 2020
Mycobacterium avium Subsp. paratuberculosis (MAP) is a gram-positive, small, acid-fast bacillus with high environmental resistance. In animals, especially ruminants, it leads to Paratuberculosis (PTB) or Johne's disease, which is chronic granulomatous ...
Nasim Sadeghi   +2 more
doaj   +1 more source

Efficiency of direct microbial diagnosis, IS900 PCR and microbial culture for detection of Mycobacterium avium subsp. Paratuberculosis in the feces of apparently healthy cattle [PDF]

open access: yesĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī, 2009
Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.
Y Anzabi   +2 more
doaj   +1 more source

Comparative evaluation of various in-house protocols on diagnostic performance for paratuberculosis IS900 PCR

open access: yesMolecular Biology Reports, 2022
Abstract Background Paratuberculosis is a worldwide endemic infectious disease of ruminants that results in high economic losses. Public health concerns are also being raised with human Crohn’s disease. Therefore, control is becoming priority for governments.
Harshita Gandhi   +5 more
openaire   +2 more sources

Mycobacterium avium subsp. paratuberculosis (MAP) en un hato lechero de búfalos en Colombia

open access: yesRevista Mvz Cordoba, 2021
Objetivo. Determinar la presencia de Mycobacterium avium subsp. paratuberculosis (MAP) mediante métodos serológicos, microbiológicos y moleculares, para genotipar los aislamientos de MAP y explorar factores asociados a la seropositividad a MAP en un hato
Brahian Camilo Tuberquia-López   +5 more
doaj   +1 more source

p43, the protein product of the atypical insertion sequence IS900, is expressed in Mycobacterium paratuberculosis [PDF]

open access: yesJournal of General Microbiology, 1992
The novel mycobacterial insertion sequence IS900 was analysed by coupled transcription-translation, of both strands independently, in a cell-free E. coli extract using an exogenous promoter. This revealed only one protein product, p43, as predicted from the nucleotide sequence. The protein was readily translated in recombinant E.
M L, Tizard   +4 more
openaire   +2 more sources

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