Results 181 to 190 of about 12,558 (256)

Isolation of human and porcine islets of langerhans and islet transplantation in pigs

Journal of Surgical Research, 1974
Abstract Using a modification of the collagenase digestion, Ficoll gradient separation technique, we isolated the islets of Langerhans from fresh human pancreata removed from braindead cadavers. Comparison of the tissue insulin/amylase ratios between whole pancreas and isolated material revealed a significant purification of islet from acinar tissue.
D E, Sutherland, M W, Steffes, G E, Bauer, D, McManus, B D, Noe, J S, Najarian   +5 more
openaire   +3 more sources

Transplantation of isolated islets of langerhans in diabetic dogs. I. Results after allogeneic intraportal islet transplantation

Journal of Surgical Research, 1979
Abstract Twenty partially inbred German shepherd dogs were made diabetic by intravenous administration of streptozotocin (25 mg/kg) 1 and 3 days after partial pancreatectomy. Ten diabetic dogs received intraportal transplants of allogeneic islets of Langerhans isolated by collagenase digestion and Ficon gradient separation.
D, Lorenz, H, Lippert, W, Tietz, V, Worm, H J, Hahn, A, Dorn, G, Koch, M, Ziegler, K D, Rosenbaum   +8 more
openaire   +3 more sources

Effect of Antithymocyte Globulin on Islet of Langerhans Transplantation

Nephron, 1978
Lewis rats were treated with streptozotocin to induce hyperglycemia and glycosuria (400-600 mg/dl). Transplantation of approximately 1,000 dissociated islets obtained from collagenase-treated pancreases from 4 donors will promptly correct induced diabetes.
E A, Friedman, M M, Beyer
openaire   +3 more sources

Islet of Langerhans Transplantation for the Treatment of Type 1 Diabetes

Swiss Surgery, 2003
Islet of Langerhans transplantation is gaining recognition as a therapy for type 1 diabetes. The procedure involves enzymatic digestion of the pancreatic tissue, purification of the islets from the exocrine tissue, infusion of the islets into the portal vein and implantation in the liver.
Bucher, Pascal Alain Robert, Mathe, Z, Bosco, Domenico, Andres, Axel, Buehler, Leo Hans, Morel, Philippe, Berney, Thierry   +6 more
openaire   +4 more sources

Multiple Transplantations of Islets of Langerhans

European Surgical Research, 1977
In 6 diabetic rats about 1,000 isolated islets of Langerhans were transplanted into the liver with little effect on the uninsulinism and hyperglycemia. Subsequently, a second transplantation resulted in a decrease of blood sugar to normal and in a reversal of the loss in body weight. Finally, in animals who had reversed to diabetes again, a third islet
K D, Rumpf, D, Löhlein, , Pichlmayr
openaire   +2 more sources

Culture of the islets of Langerhans for transplantation

The Japanese Journal of Surgery, 1982
The isolated islets of Langerhans are the most available donors for transplantation. As the preservation of the isolated islets is difficult, we attempted to keep these tissues viable by use of an organ culture. Islets of Langerhans from adult Wistar rats were isolated by a collagenase technique and cultured in air-CO2 (95-5%) incubator at 37 degrees C.
Y, Kojima, G, Nakagawara, I, Miyazaki
openaire   +2 more sources

Bovine Islets of Langerhans: Potential Source for Transplantation?

Diabetes, 1989
Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,
B J, Hering, D, Romann, A, Clarius, M, Brendel, M, Slijepcevic, R G, Bretzel, K, Federlin   +6 more
openaire   +2 more sources

Suppression, Stress, and Accommodation of Transplanted Islets of Langerhans

Diabetes, 1985
Successful intrasplenic islet autotransplantation in dogs requires an islet cell mass considerably greater than what might be expected based on studies of subtotal pancreatectomy. Grafts of marginal function ultimately fail, suggesting severe limitations in the capacity of an islet graft to adapt.
R C, Merrell, M, Maeda, G, Basadonna, F, Marincola, L, Cobb   +4 more
openaire   +2 more sources

Transplantation of islets of Langerhans

Journal of Hepato-Biliary-Pancreatic Surgery, 1994
The present study attempts to evaluate the possibility of using cryopreserved pancreatic islets in rats. Insulin-releasing activity was studied for the purpose of clarifying the function of cryopreserved islets. Cryopreserved pancreatic islets were also transplanted into the portal veins of recipient rats.
openaire   +1 more source

Transplantation of isologous islets of langerhans in diabetic rats

Acta Diabetologica Latina, 1975
Isologous isolated islets of Langerhans were transplanted into the peritoneum and, via the portal vein, into the liver of diabetic rats. In both groups almost normal blood glucose and serum insulin levels were achieved for a period of three months. Glucose tolerance tests were markedly improved.
D, Lorenz, K D, Rosenbaum, J, Petermann, M, Ziegler, R, Beckert, A, Dorn   +5 more
openaire   +2 more sources