Solvent isotope-induced equilibrium perturbation for isocitrate lyase. [PDF]
Biochemistry, 2013 Isocitrate lyase (ICL) catalyzes the reversible retro-aldol cleavage of isocitrate to generate glyoxylate and succinate. ICL is the first enzyme of the glyoxylate shunt, which allows for the anaplerosis of citric acid cycle intermediates under nutrient limiting conditions. In Mycobacterium tuberculosis, the source of ICL for these studies, ICL is vital
Quartararo CE +3 more
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Heterogeneous multimeric structure of isocitrate lyase in complex with succinate and itaconate provides novel insights into its inhibitory mechanism. [PDF]
PLoS One, 2021 During the glyoxylate cycle, isocitrate lyases (ICLs) catalyze the lysis of isocitrate to glyoxylate and succinate. Itaconate has been reported to inhibit an ICL from Mycobacterium tuberculosis (tbICL).
Kwon S, Chun HL, Ha HJ, Lee SY, Park HH.
europepmc +2 more sources
Inhibition of Paracoccidioides lutzii Pb01 isocitrate lyase by the natural compound argentilactone and its semi-synthetic derivatives. [PDF]
PLoS ONE, 2014 The dimorphic fungus Paracoccidioides spp. is responsible for paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America, causing serious public health problems.
Renata Silva do Prado +8 more
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Itaconate is a covalent inhibitor of the Mycobacterium tuberculosis isocitrate lyase. [PDF]
RSC Med Chem, 2021 Itaconate is a mammalian antimicrobial metabolite that inhibits the isocitrate lyases (ICLs) of Mycobacterium tuberculosis. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue.
Kwai BXC +5 more
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Physiological role of isocitrate lyase in dibenzo-p-dioxin and dibenzofuran metabolism by Sphingomonas wittichii RW1 [PDF]
Journal of Genetic Engineering and Biotechnology, 2022 Background Sphingomonas wittichii RW1 is one out of three strains capable of metabolizing dioxin as a sole source for carbon and energy. Under laboratory conditions the degradation rates for these aromatics are relatively high (5 and 8 h for dibenzofuran
Rayan M. Faisal, Aveen H. Rasol
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Isocitrate Lyase from Flax [PDF]
Plant Physiology, 1982 The cleavage of D(s)-isocitrate catalyzed by isocitrate lyase from Linum usitatissimum results in the ordered release of succinate and glyoxylate. The glyoxylate analog 3-bromopyruvate irreversibly inactivates the flax enzyme in a process exhibiting saturation kinetics and protection by glyoxylate or isocitrate or the competitive inhibitor l-tartrate ...
F R, Khan, B A, McFadden
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Modulation of Central Carbon Metabolism by Acetylation of Isocitrate Lyase in Mycobacterium tuberculosis. [PDF]
Sci Rep, 2017 Several enzymes involved in central carbon metabolism such as isocitrate lyase and phosphoenolpyruvate carboxykinase are key determinants of pathogenesis of Mycobacterium tuberculosis (M. tb).
Bi J +7 more
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Distant Phe345 mutation compromises the stability and activity of Mycobacterium tuberculosis isocitrate lyase by modulating its structural flexibility. [PDF]
Sci Rep, 2017 Isocitrate lyase (ICL), a potential anti-tubercular drug target, catalyzes the first step of the glyoxylate shunt. In the present investigation, we studied the conformational flexibility of MtbICL to better understand its stability and catalytic activity.
Shukla H +4 more
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Inhibitory Effects of Nitrogenous Metabolites from a Marine-Derived Streptomyces bacillaris on Isocitrate Lyase of Candida albicans [PDF]
Marine Drugs, 2022 Two nitrogenous metabolites, bacillimide (1) and bacillapyrrole (2), were isolated from the culture broth of the marine-derived actinomycete Streptomyces bacillaris.
Beomkoo Chung +9 more
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Two distinct isocitrate lyases from a pseudomonas species [PDF]
Journal of Bacteriology, 1975 The isocitrate lyases of acetate- and methylamine-grown Pseudomonas MA (Shaw strain) were studied. They were shown to be different by a variety of physical criteria including chromatographic elution patterns, heat inactivation kinetics, pH variation of Km values, and migration on polyacrylamide gels.
E, Bellion, J, Woodson
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