Results 171 to 180 of about 35,027 (292)

Isoelectric focusing of urinary metallothionein.

, 1990
Isoelectric focusing of human urinary metallothionein at a pH range of 4.8 to 7.0 yielded a single protein band with a pI of 5.57 which co-migrated with authentic purified metallothionein I from human liver. Minimum pretreatment of the urine samples (160
Felley-Bosco, E., Hunziker, P.E., Savolainen, H.   +2 more
core  

Alliinase: A Comprehensive Review of the Pivotal Enzyme in Garlic Chemistry and Its Applications

Food Frontiers, Volume 7, Issue 4, July 2026.
Garlic crude extract was purified to obtain alliinase, which catalyzes alliin hydrolysis to release allicin. The free and immobilized alliinase shows potential applications in food, biotechnology, pharmaceuticals, health products, and biosensors. ABSTRACT Alliinase is a pyridoxal 5′‐phosphate (PLP)‐dependent lyase that serves as the cornerstone of the ...
Zhenlin Han, Tian Ye, Yantian Tang, Guodong Su, Jiazhou Li, Ningyang Li   +5 more
wiley   +1 more source

On-chip microscale isoelectric focusing enhances protein detection limit. [PDF]

Appl Phys Lett
Fan X, Zhang X, Bao H, Zhang X, Ping J.
europepmc   +1 more source

Isoelectric Focusing [PDF]

, 2020
openaire   +1 more source

Interactions of Plant Protein and Natural Colorant: A Structural and Functional Relationship of Broccoli Protein and Marigold Color Compounds

Food Frontiers, Volume 7, Issue 4, July 2026.
Graphical representation of the interactions of the structural and functional relationships of broccoli flower protein (BFP) and marigold color compounds (MCC). This research examines BFP–MCC interactions of extracted marigold color, chemical composition, and functional attributes.
Fidele Benimana, Kentaro Kawata, Anand Mohan   +2 more
wiley   +1 more source

Isoelectric Focusing Fractionation Method for Signal Enhancement in Detection of Inactivated Biological Agents Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. [PDF]

Electrophoresis
Duša F, Šalplachta J, Horká M, Lunerová K, Čermáková V, Dřevínek M, Kubíček O.   +6 more
europepmc   +1 more source

Isoelectric focusing: Sample pretreatment – separation – hyphenation

, 2009
The three major goals of the research presented in this thesis are: • Investigation of electrophoretic sample pretreatment strategies. • Study and optimization of capillary isoelectric focusing for separation of proteins.
Farmaceutische Analyse, Dep Farmaceutische wetenschappen, Silvertand, L.H.H.   +2 more
core  

Using generalized quantitative structure–property relationship (QSPR) models to predict host cell protein retention in ion‐exchange chromatography

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 7, Page 1420-1428, July 2026.
Abstract BACKGROUND Selecting an optimal chromatography resin during biopharmaceutical downstream process development is a great challenge. This is especially the case for recombinant subunit vaccines, where product properties vary greatly and recovery often involves cell lysis, which yields a complex mixture of different host cell materials. Host cell
Tim Neijenhuis, Olivier Le Bussy, Geoffroy Geldhof, Marieke E Klijn, Marcel Ottens   +4 more
wiley   +1 more source

[Rapid identification and analysis of hemoglobin isoelectric focusing electrophoresis images based on deep learning]. [PDF]

Se Pu
Ji WC, Tian YL, Fu HD, Zha GH, Cao CX, Wei L, Zhang Q.   +6 more
europepmc   +1 more source

Optimizing rapeseed protein purification: a continuous chromatographic approach for napin and cruciferin

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 7, Page 1370-1383, July 2026.
Abstract BACKGROUND Napin and cruciferin, the predominant storage proteins in Brassica napus (rapeseed), exhibit favorable techno‐functional properties such as emulsification and gel formation, positioning them as promising candidates for the encapsulation and delivery of bioactive compounds. Their classification as GRAS (Generally Recognized As Safe),
Jonas Arnecke, Jessica Gillmann, Tim Börner, Mathias Hafner, Christian Frech   +4 more
wiley   +1 more source