Results 161 to 170 of about 60,313 (296)

Harnessing Macromolecular Crowding of Proteins for Engineering Sustainable Nanofiltration Membranes

SusMat, EarlyView.
Novel biodegradable and solvent‐resistant nanofiltration membranes fabricated by harnessing the macromolecular crowding of zein protein induced by solvent interactions. Scattering experiments and molecular dynamics simulations confirmed the macromolecular crowding formation and revealed the structural characteristics of the membranes.
Claudia Oviedo, Diana G. Oldal, Rifan Hardian, Tibor Holtzl, Maged F. Serag, Satoshi Habuchi, Gyorgy Szekely   +6 more
wiley   +1 more source

Crystal structure and functional characterization of an Asp49 phospholipase A2 from the bushmaster (Lachesis muta)

Acta Crystallographica Section F, EarlyView.
The crystal structure of an Asp49 phospholipase A2 from L. muta is reported, revealing a tetrameric assembly, catalytic conservation and a polarized electrostatic surface potentially linked to membrane targeting.Snake‐venom phospholipases A2 (PLA2s) are small, structurally conserved enzymes that contribute significantly to the pathophysiology of ...
Noelia Erika Neyra Chama, Frey Francisco Romero Vargas, Eloy Condori Mamani, Jhon Antoni Vargas, Adriano Alves Furtado, Humberto D'Muniz Pereira, Ronald Demetrio Navarro Oviedo, Richard Charles Garratt, José Luis Javier Vega Ramírez, Diego Antonio Leonardo   +9 more
wiley   +1 more source

Silencing of juvenile hormone‐related genes through RNA interference leads to molt failure and high mortality in the spongy moth

Insect Science, EarlyView.
The feasibility of using RNA interference to control the globally important quarantine pest, the spongy moth. Targeting genes related to JHs play an important role in the growth and development of insects. First, the open reading frames (ORFs) of Ldjhamt and Ldjheh were identified and characterized, and the target genes were cloned and double‐stranded ...
Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi   +3 more
wiley   +1 more source

Automated Capillary Isoelectric Focusing-Tandem Mass Spectrometry for Qualitative and Quantitative Top-Down Proteomics. [PDF]

Anal Chem, 2020
Xu T, Shen X, Yang Z, Chen D, Lubeckyj RA, McCool EN, Sun L.   +6 more
europepmc   +1 more source

LsFAMeT and LsJHAMT coordinate JH biosynthesis to regulate fecundity in Laodelphax striatellus

Insect Science, EarlyView.
The small brown planthopper (SBPH), Laodelphax striatellus, can trigger large‐scale outbreaks when encountering suitable habitats during migration due to their strong reproductive ability. This study investigated the role of juvenile hormone (JH) in regulating SBPH reproduction.
Yan Guo, Bing‐Bing Shi, Xue‐Qi Zhang, Qiong Yang   +3 more
wiley   +1 more source

TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum

Insect Science, EarlyView.
RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li, Jie Chen, Zi‐Ye Wang, Fei‐Feng Wang, Lu‐Lu Dong, Jun Pan, Min‐Er Li, Lin Yu, Wen Sang   +8 more
wiley   +1 more source

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method. [PDF]

J Vis Exp, 2020
Veerapandian R, Paudyal A, Chang A, Vediyappan G.   +3 more
europepmc   +1 more source

Genome‐wide analysis of DNA methyltransferases in a model extremophyte, Schrenkiella parvula: Transcriptional dynamics during development and under salt stress

Annals of Applied Biology, EarlyView.
Classification of DMTs into 4 subfamilies: two METs, three CMTs, three DRMs, and one DNMT2. The cis‐elements are associated with stress‐response, hormonal regulation, light‐response, and development. RNA‐seq analysis revealed differential expression of DMTs under NaCl stress.
Seher Yolcu, Amin Mirshamsi Kakhki, Ahmad Jalilian, Emre Sevindik, Ismail Turkan   +4 more
wiley   +1 more source

Ion-exchange chromatography, capillary isoelectric focusing, and capillary zone electrophoresis coupled to mass spectrometry for charge variant analysis of monoclonal antibodies. [PDF]

MAbs
Schairer J, Höchsmann A, Bauer B, Höfer V, Römer J, Neusüß C.   +5 more
europepmc   +1 more source

Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus

Allergy, EarlyView.
This study established a microarray containing a representative panel of purified Blo t and Der p allergens for the identification of patients sensitised to Blo t and/or Der p. Results indicate that the microarray based on Blo t and Der p allergens is a useful tool for the identification of Blo t‐ and/or Der p‐sensitised patients. The microarray may be
Nishelle Dsouza, Siratcha Phanthong, Huey‐Jy Huang, Milena Weber, Eszter Sarzsinszky, Petra Zieglmayer, Gabrielle Pauli, Margarete Focke‐Tejkl, Mikhail Tulaev, Nikolina Todorovic, Walter Keller, Thomas Schlederer, Luis Caraballo, Anchalee Tungtrongchitr, Rudolf Valenta, Pongsakorn Tantilipikorn, Susanne Vrtala   +16 more
wiley   +1 more source