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Isoelectric focusing in polyacrylamide gels
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971From many viewpoints, isoelectric focusing (IEF) represents a major advance in methodology for high-resolution separation of proteins and other amphoteric macromolecules. IEF is essentially an equilibrium method for segregating amphoteric molecules by electrophoresis in stable pH gradients according to their isoelectric points (pI).
P, Righetti, J W, Drysdale
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Two‐dimensional gel isoelectric focusing
ELECTROPHORESIS, 2005AbstractTwo‐dimensional gel isoelectric focusing (2‐D gel IEF) is presented as the combination of the same separation method used consecutively in two directions of the same gel. In this new method, after completion of IEF process in the first dimension the gel was cut into the separate strips, each containing selected analytes together with the ...
Miroslava, Stastná, Karel, Slais
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Capillary isoelectric focusing
2013Capillary isoelectric focusing (CIEF) is a widespread technique for the analysis of peptides and proteins in biological samples. CIEF is used to separate mixtures of compounds on the basis of differences in their isoelectric point. Aspects of sample preparation, capillary selection, zone mobilization procedures as well as various detection modes used ...
Bujak, Renata, Daghir, Emilia
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Preparative isoelectric focusing in agarose
Analytical Biochemistry, 1981Abstract The use of agarose gels as supporting media for flat-bed preparative isoelectric focusing was applied to the fractionation of serum proteins in the pH range 3.5–6, and red cell hemolysates in the pH range 3–8. The agarose gels are easy to prepare, give linear pH gradients, and do not appear to produce molecular sieving effects.
C, Chapuis-Cellier, P, Arnaud
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Preparative Isoelectric Focusing
Separation and Purification Methods, 1975Abstract Electrophoretic mobility has been used for years as a basis for separation and characterization of proteins. The resolving power of conventional electrophoresis is limited, and the characterization by mobility is not unique because of the dependence of the protein mobilities on the pH and ionic strength of the buffers.
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1990
Publisher Summary Isoelectric focusing (IEF) is a technique that was developed to separate proteins on the basis of differences in their isoelectric pH value, termed “pI values.” It is used for both the analysis and preparative isolation of proteins. IEF is an electrophoretic method in which amphoteric molecules are separated as they migrate through ...
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Publisher Summary Isoelectric focusing (IEF) is a technique that was developed to separate proteins on the basis of differences in their isoelectric pH value, termed “pI values.” It is used for both the analysis and preparative isolation of proteins. IEF is an electrophoretic method in which amphoteric molecules are separated as they migrate through ...
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
exaly
Isoelectric focusing of proteolipid
Journal of Neurochemistry, 1978M, Draper, M B, Lees, D S, Chan
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