Results 251 to 260 of about 35,027 (292)

Improvements of isoelectric focusing in agarose for direct tissue isoelectric focusing

ELECTROPHORESIS, 1982
AbstractImprovements of isoelectric focusing (IEF) in agarose are described that result in consistently obtaining broad, linear pH gradients in the range pH 4 to 9.2 at equilibrium. Cathodic drift has been reduced by the use of a blend of commercial carrier ampholytes and by incorporating lysine and arginine in the catholyte as spacers.
Basil J. Thompson, Michael J. Dunn, Arthur H. M. Burghes, V. Dubowitz   +3 more
openaire   +1 more source

Preparative isoelectric focusing in agarose

Analytical Biochemistry, 1981
Abstract The use of agarose gels as supporting media for flat-bed preparative isoelectric focusing was applied to the fractionation of serum proteins in the pH range 3.5–6, and red cell hemolysates in the pH range 3–8. The agarose gels are easy to prepare, give linear pH gradients, and do not appear to produce molecular sieving effects.
C, Chapuis-Cellier, P, Arnaud
openaire   +2 more sources

ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS

Australian Journal of Experimental Biology and Medical Science, 1970
SummaryAn improved technique for isoelcctric focusing of proteins in polyacrylamide gels is described.In studying insulin with the procedure, mercuric chloride in trichloro‐acetic acid was used to fix the protein in the gel.One major and five minor components were demonstrated in ox and pig insulin.
L H, Percival, G G, Dunckley, H D, Purves   +2 more
openaire   +2 more sources

Isoelectric Focusing of Peripheral Lymphocytes

Preparative Biochemistry, 1974
Abstract The technique of isoelectric focusing has been applied to the differential separation of peripheral lymphocytes of both human and rabbit origin. It has been shown to provide subpopulations of lymphocytes that may be used for further biological and immunochemical studies.
E M, Leise, F, LeSane
openaire   +2 more sources

The transitional isoelectric focusing process

Analytical and Bioanalytical Chemistry, 2004
The transitional isoelectric focusing (IEF) process (the course of pH gradient formation by carrier ampholytes (CAs) and the correlation of the focusing time with CA concentration) were investigated using a whole-column detection capillary isoelectric focusing (CIEF) system.
Tiemin, Huang, Zhen, Liu, Janusz, Pawliszyn   +2 more
openaire   +2 more sources

Isoelectric focusing of carboxypeptidase N

Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
Carboxypeptidase N was partially purified on a TEAE-cellulose column and subjected to isoelectric focusing in sucrose gradient columns containing ampholine gradients of pH range 3-10 and 4-8. Activity separated into two major peaks with pI values of pH 3.8 and 4.3.
A, Koheil, G, Forstner
openaire   +2 more sources

Isoelectric focusing in layers of granulated gels II. Preparative isoelectric focusing

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1975
A method for preparative isoelectric focusing of 0.1-10 g amounts of proteins is described. For anticonvective stabilization of the pH gradient, layers of granulated gels (E.G. Sephadex or Bio-Gel) of variable length, width and thickness were used either on glass plates or in troughs.
openaire   +2 more sources

Isoelectric focusing in polyacrylamide gels

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971
From many viewpoints, isoelectric focusing (IEF) represents a major advance in methodology for high-resolution separation of proteins and other amphoteric macromolecules. IEF is essentially an equilibrium method for segregating amphoteric molecules by electrophoresis in stable pH gradients according to their isoelectric points (pI).
P, Righetti, J W, Drysdale
openaire   +2 more sources

Recycling isoelectric focusing and isotachophoresis

ELECTROPHORESIS, 1998
AbstractOf all electrophoretic methods, isoelectric focusing offers the highest resolution and is best suited for preparative applications. Over the years, several instruments were developed for this purpose, all operating in free fluids, in the absence of gels or other supporting matrices.
openaire   +2 more sources

Isoelectric focusing of proteolipid

Journal of Neurochemistry, 1978
M, Draper, M B, Lees, D S, Chan
openaire   +2 more sources