Results 241 to 250 of about 60,313 (296)
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Parallel isoelectric focusing chip
PROTEOMICS, 2004AbstractFast isoelectric focusing (IEF) is becoming a key method in modern protein analysis. We report here the theory and experimental results of new parallel isoelectric devices (PID) for fast IEF. The main separation tool of any PID is a dielectric membrane with conducting channels filled by immobiline gels of varying pH.
Gleb, Zilberstein +3 more
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Journal of Chromatography A, 1974
Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Isoelectric focusing in agarose
Journal of Immunological Methods, 1979We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused.
C A, Saravis, N, Zamcheck
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Methods of isoelectric focusing
American Journal of Physical Anthropology, 1979AbstractThe method of isoelectric focusing has been avoided by many workers because of expense, technical difficulty, and problems of interpretation. Inexpensive, easy, and interpretable results are possible using equipment and reagents commonly available. Methods which allow these results are presented and explained.
R H, Byles, W, McGuire, M F, Sanders
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Isoelectric focusing of neuraminidase
Experientia, 1970Die isoelektrischen Punkte der Neuraminidasen vonVibrio cholerae (pH 4.80) undClostridium perfringens (pH 4.95) wurden mittels isoelektrischer Fokusierung bestimmt. Neuraminidasen zwei verschiedener Influenzaviren (A2/Aichi/68 und B/Mass/66) wurden entsprechend analysiert.
A R, Neurath, R W, Hartzell, B A, Rubin
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Parallel isoelectric focusing II
ELECTROPHORESIS, 2004AbstractA miniature electrophoretic device is developed on the basis of a new isoelectric focusing (IEF) method, namely parallel isoelectric focusing. We report here the theory and the results of operation of a new parallel isoelectric device (PID). The main advantages and limitations of the method are discussed for miniaturization purposes.
Gleb V, Zilberstein +3 more
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Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing
Journal of Biochemical and Biophysical Methods, 1987Amphoteric, isoelectric agarose membranes, as devised by Martin and Hampson [Martin, A.J.P. and Hampson, F. (1978) J. Chromatogr. 159, 101-110], are found unsuitable for blocking electroendosmosis in multi-compartment electrolysers during preparative isoelectric focusing, due to the poor and highly unpredictable incorporation of carboxyls and amino ...
P, Wenger +4 more
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Isoelectric focusing without ampholyte
Biochemical and Biophysical Research Communications, 1979Summary We have studied the electrophoretic mobility of proteins in polyacrylamide gels when ampholyte and buffer were excluded from the system. We found, using a weak acid and base as anolyte and catholyte respectively, that a pH gradient was generated in the gel, and that proteins focused as sharp bands.
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Divergent flow isoelectric focusing
ELECTROPHORESIS, 2008AbstractContinuousâflow isoelectric focusing (IEF) has the potential to be an important method in proteome analysis. The current devices do not fully use the advantages of IEF, because they do not utilize all its important features including changes in background conductivity during the focusing.
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Isoelectric focusing in layers of granulated gels II. Preparative isoelectric focusing
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1975A method for preparative isoelectric focusing of 0.1-10 g amounts of proteins is described. For anticonvective stabilization of the pH gradient, layers of granulated gels (E.G. Sephadex or Bio-Gel) of variable length, width and thickness were used either on glass plates or in troughs.
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