Results 241 to 250 of about 35,027 (292)
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Capillary isoelectric focusing
Electrophoresis, 1997AbstractCapillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability.
Tim Wehr, Mingde Zhu
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Isoelectric focusing in a Sephadex column
Analytical Biochemistry, 1976 Abstract An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5–10 reaches equilibrium in approximately 5 hr and thereby reduces the ...
T J, O'Brien +3 more
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Isoelectric focusing electrophoresis of lignin
Analytical Biochemistry, 1991 Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components ...
Niku-Paavola, Marja-Leena
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ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS
Lancet, The, 1968G Dale, A L Latner
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Journal of Chromatography A, 1974
Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Isoelectric focusing in agarose
Journal of Immunological Methods, 1979We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused.
C A, Saravis, N, Zamcheck
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Methods of isoelectric focusing
American Journal of Physical Anthropology, 1979AbstractThe method of isoelectric focusing has been avoided by many workers because of expense, technical difficulty, and problems of interpretation. Inexpensive, easy, and interpretable results are possible using equipment and reagents commonly available. Methods which allow these results are presented and explained.
R H, Byles, W, McGuire, M F, Sanders
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Isoelectric focusing of neuraminidase
Experientia, 1970Die isoelektrischen Punkte der Neuraminidasen vonVibrio cholerae (pH 4.80) undClostridium perfringens (pH 4.95) wurden mittels isoelektrischer Fokusierung bestimmt. Neuraminidasen zwei verschiedener Influenzaviren (A2/Aichi/68 und B/Mass/66) wurden entsprechend analysiert.
A R, Neurath, R W, Hartzell, B A, Rubin
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Isoelectric focusing in polyacrylamide-agarose
Analytical Biochemistry, 1984A procedure using a 2.5% acrylamide-0.5% agarose gel for slab or tube isoelectric focusing is described. This composite gel is durable and enables a rapid focusing of high-molecular-weight compounds.
R M, Pino, T K, Hart
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Parallel isoelectric focusing chip
PROTEOMICS, 2004AbstractFast isoelectric focusing (IEF) is becoming a key method in modern protein analysis. We report here the theory and experimental results of new parallel isoelectric devices (PID) for fast IEF. The main separation tool of any PID is a dielectric membrane with conducting channels filled by immobiline gels of varying pH.
Gleb, Zilberstein +3 more
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