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Isoelectric focusing and isoelectric points of bovine liver histones
Analytical Biochemistry, 1980Abstract A technique for isoelectric focusing of total histones in very narrow pH gradients is described. The isoelectric focusing was performed in 5% acrylamide gels at the pH range 9–11 in long quartz tubes (24 cm) in a nitrogen atmosphere. The total bovine liver histones separated into five main fractions which were identified as H1, H3, H2B, H2A,
K H, Valkonen, R S, Piha
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Virus isoelectric point determination using single-particle chemical force microscopy.
Langmuir, 2019Virus colloidal behavior is governed by the interaction of the viral surface and the surrounding environment. One method to characterize the virus surface charge is the isoelectric point (pI). Traditional determination of virus pI has focused on the bulk
X. Mi +4 more
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Isoelectric points of proteins: Theoretical determination
Analytical Biochemistry, 1989Three methods for calculating the isoelectric points (pI) of proteins, provided that their amino acid compositions are known, are described. The comprehensive and abridged procedures involve solutions of polynomial equations of different degrees depending on whether pK values of the specific acid-base residues or the means of some of those values ...
A, Sillero, J M, Ribeiro
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ACS Sustainable Chemistry and Engineering, 2018
Anaerobic bioconversion of waste activated sludge (WAS) into methane is often limited by the slow hydrolysis rate and/or poor methane potential. In this study, a novel pretreatment strategy based on the isoelectric point of sludge particulate is proposed
Ying Xu +3 more
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Anaerobic bioconversion of waste activated sludge (WAS) into methane is often limited by the slow hydrolysis rate and/or poor methane potential. In this study, a novel pretreatment strategy based on the isoelectric point of sludge particulate is proposed
Ying Xu +3 more
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Isoelectric points of proteins: A table
Analytical Biochemistry, 1978We have prepared a table of isoelectric points (pZ) of proteins selected from the literature. Initially, we retrieved a computerized bibliography from the MEDLARS data base covering the time period of January 1970-March 1976. This search, supplemented by an additional search of Biological Abstracts, produced about 3000 citations. From this bibliography
D, Malamud, J W, Drysdale
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The isoelectric point of Cypridina luciferase
Archives of Biochemistry and Biophysics, 1955Abstract Measurements of the mobility of Cypridina luciferase by means of paper electrophoresis at seven pH values between 2.38 and 6.18 indicate an apparent isoelectric point of 3.28. The reliability of this value is enhanced by the close agreement found between the isoelectric point of bovine plasma albumin determined by paper electrophoresis and
J H, WEIR, F I, TSUJI, A M, CHASE
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Method for Estimation of Protein Isoelectric Point
Analytical Chemistry, 2012Adsorption of sample protein to Eu(3+) chelate-labeled nanoparticles is the basis of the developed noncompetitive and homogeneous method for the estimation of the protein isoelectric point (pI). The lanthanide ion of the nanoparticle surface-conjugated Eu(3+) chelate is dissociated at a low pH, therefore decreasing the luminescence signal.
Pihlasalo, Sari +3 more
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Point of Zero Charge and Isoelectric Point of Alumina
Materials and Manufacturing Processes, 2008The point of zero charge, pHpzc, and the isoelectric point, pHiep, of a commercial alumina sample was investigated in KNO3 solutions. The influence of the concentration and the initial pH value of KNO3 solution used for preparation and equilibration of the alumina suspension, as well as of the alumina/solution ratio on pHpzc and pHiep were investigated.
Gulicovski, Jelena J. +2 more
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