Results 11 to 20 of about 5,260,747 (202)
Horticulturae, 2022 Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation ...
Elena V. Kozar +2 more
doaj +3 more sources
EMBRYOGENESIS IN CULTURE OF ISOLATED MICROSPORE OF BROCCOLI [PDF]
Овощи России, 2018 The process of embryogenesis and technological experimental protocol has been studied and applied to produce doubled haploid plants from microspore cultured in vitro in broccoli B. oleracea L. convar. botrytis (L.) Alef. var. italica Plenck. It was shown
E. A. Domblides +5 more
doaj +4 more sources
Isolated Microspore Culture in Barley
, 2018 Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential.
Esteves, Patricio, Belzile, François
openaire +4 more sources
Plants, 2020 Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed.
Daria Shumilina +4 more
doaj +2 more sources
PRODUCTION OF DH-PLANTS IN CULTURE OF ISOLATED MICROSPORE IN CARROT
Овощи России, 2018 The main goals of the research were to study the factors affecting on the process of embryogenesis in culture of isolated microspores, optimize the existing protocols, and finally produce the doubled haploid plants in carrot (DH-plants). Out of 10 carrot
T. S. Vjurtts +5 more
doaj +3 more sources
Microspore embryogenesis in vitro: the role of stresses [PDF]
Вавиловский журнал генетики и селекции, 2019 Gametic embryogenesis is one form of totipotency of plant cells, in which either male or female gametes are induced to form embryoids (sporophytes). Regeneration of haploid plants from embryoids and subsequent chromosome duplication result in doubled ...
T. I. Djatchouk +4 more
doaj +2 more sources
Effects of culture conditions on isolated microspore culture of melon (Cucumis melo L.)
Vietnam Journal of Science, Technology and Engineering, 2023 Isolated microspore culture is a useful tool to produce pure, fully homozygous parental lines in a short time. This study evaluated factors including the microspore developmental stage, cell culture density, and heat treatment influencing callus ...
Minh Ly Nguyen, Ton Nu Bao Tien Huyen
doaj +1 more source
In Vitro Live Cell Imaging Reveals Nuclear Dynamics and Role of the Cytoskeleton During Asymmetric Division of Pollen Mitosis I in Nicotiana Benthamiana. [PDF]
Cytoskeleton (Hoboken)ABSTRACT Pollen is a male gametophyte of angiosperms. Following meiosis, the microspore undergoes an asymmetric division called pollen mitosis I (PMI), which produces two cells of different sizes: a large vegetative cell and a small generative cell. Polarized nuclear migration and positioning during PMI are important for successful pollen development ...
Mizuta Y +5 more
europepmc +2 more sources
Improvement of microspore culture method for multiple samples in Brassica [PDF]
, 2011 Microspore culture is an important method for production of haploids and doubled haploids. Although the routine protocol of isolated microspore culture of Brassica species has been established, the protocol for a large number of genotypes is laborious ...
Takahashi, Yu +2 more
core +2 more sources