Results 121 to 130 of about 333,047 (329)

Decoding Blastocystis‐Driven Mechanisms in Gut Microbiota and Host Metabolism

Advanced Science, EarlyView.
Blastocystis, a prevalent gut microbe, interacts with the host through metabolic and immune pathways, influencing cardiometabolic health. This perspective explores its role in modulating short‐chain fatty acid production, tryptophan metabolism, and immune signaling, potentially impacting cholesterol absorption, glucose metabolism, and vascular health ...
Lei Deng, Kevin SW Tan
wiley   +1 more source

Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage

Luminescence, Volume 37, Issue 12, Page 2129-2138, December 2022., 2022
Bacterial mineralization treatments can be effective, nontoxic, environmentally friendly, and chemically compatible with fossil heritage. However, accurate monitoring should be carried out to detect bacteria viability during and after treatments. Adenosine triphosphate (ATP) assay is proposed as a fast, affordable, portable, and easy‐to‐use system for ...
Silvia Marín‐Ortega, M. Àngels Calvo i Torras, Manuel Ángel Iglesias‐Campos   +2 more
wiley   +1 more source

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

STAR Protocols
Summary: A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids.
Miner Deng, Yanting Wei, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo   +5 more
doaj  

Host Plasma Microenvironment in Immunometabolically Impaired HIV Infection Leads to Dysregulated Monocyte Function and Synaptic Transmission Ex Vivo

Advanced Science, EarlyView.
Network‐based risk stratification of people living with HIV (PWH) on prolonged therapy using multi‐omics data identifies 44% of the patients are at risk of immunometabolic complications. A senescence‐associated myeloid cell‐driven plasma microenvironment drives immunometabolic dysregulation in at‐risk PWH.
Flora Mikaeloff, Marco Gelpi, Alejandra Escós, Tianqi Wang, Soham Gupta, Anna Olofsson, Sara Svensson Akusjärvi, Sabrina Schuster, Prajakta Naval, Vikas Sood, Negin Nikouyan, Andreas D. Knudsen, Beate Vestad, Julie Høgh, Johannes R. Hov, Thomas Benfield, Marius Trøseid, Vinay Pawar, Marijana Rucevic, Rui Benfeitas, Ákos Végvári, Liam O'Mahony, Rajkumar Savai, Niklas K. Björkström, Magda Lourda, João Pedro de Magalhães, Siegfried Weiss, Adil Mardinoglu, Mukesh Kumar Varshney, Annika C. Karlsson, Yasir Ahmed Syed, Susanne D. Nielsen, Ujjwal Neogi   +32 more
wiley   +1 more source

Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses

STAR Protocols
Summary: Air-liquid interface (ALI) culture can differentiate airway epithelial cells to recapitulate the respiratory tract in vitro. Here, we present a protocol for isolating and culturing nasal epithelial cells from turbinate tissues for severe acute ...
Victor H.K. Lam, Aleena Ghafoor, Yazan Khan, Shirley Constable, Lane B. Buchanan, David Zuanazzi, Reeya Parmar, Zeynep G. Tepe, Leigh J Sowerby, Cindy M. Liu, Ryan M. Troyer, Jessica L. Prodger   +11 more
doaj  

Microbial and Viral Ecology Analysis for Metagenomic Data [PDF]

arXiv
The explosion in known microbial diversity in the last two decades has made it abundantly clear that microbes in the environment do not exist in isolation; they are members of communities. Accordingly, omics approaches such as metagenomics have revealed that interactions between diverse groups of community members such as archaea, bacteria, and viruses
arxiv  

Text-mining and ontologies: new approaches to knowledge discovery of microbial diversity [PDF]

Proceedings of the 4th International Microbial Diversity Conference. pp. 221-227, ed. Marco Gobetti. Pub. Simtra. ISBN 978-88-943010-0-7, Bari, October 2017, 2018
Microbiology research has access to a very large amount of public information on the habitats of microorganisms. Many areas of microbiology research uses this information, primarily in biodiversity studies. However the habitat information is expressed in unstructured natural language form, which hinders its exploitation at large-scale.
arxiv  

Single‐Cell and Spatial Transcriptomics Reveals a Stereoscopic Response of Rice Leaf Cells to Magnaporthe oryzae Infection

Advanced Science, EarlyView.
By employing a combination of single cell and spatial transcriptomic sequencing, this study presents a stereoscopic response of rice leaf to Magnaporthe oryzae infection. The vascular tissues mount defenses by producing phytoalexins. The immune strength is stronger toward the rice leaf tip than that of the leaf base.
Wei Wang, Xianyu Zhang, Yong Zhang, Zhe Zhang, Chang Yang, Wen Cao, Yuqin Liang, Qinzheng Zhou, Qian Hu, Yimai Zhang, Yu Wang, Yingying Xing, Wenfeng Qian, Nan Yao, Ning Xu, Jun Liu   +15 more
wiley   +1 more source

Design and optimization of membrane chromatography for monoclonal antibody charge variant separation

Biotechnology Progress, Volume 38, Issue 6, November/December 2022., 2022
Abstract The manufacturing scale implementation of membrane chromatography to purify monoclonal antibodies has gradually increased with the shift in industry focus toward flexible manufacturing and disposable technologies. Membrane chromatography are used to remove process‐related impurities such as host cell proteins (HCPs) and DNA, leachates, and ...
Sathish Nadar, Balaji Somasundaram, Marcela Charry, Jagan Billakanti, Evan Shave, Kym Baker, Linda H. L. Lua   +6 more
wiley   +1 more source

Analyzing human CD4+ T cells activated in response to macrophages infected with Mycobacterium tuberculosis

STAR Protocols
Summary: M1- and M2-like macrophages infected with Mycobacterium tuberculosis (Mtb) have been found to differ in their capacity to elicit memory CD4+ T cell activation. Here, we present a protocol to quantify and isolate the subset of human memory CD4+ T 
Daniel P. Gail, Vinicius G. Suzart, Stephen M. Carpenter   +2 more
doaj