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Single-cell analysis and isolation for microbiology and biotechnology: methods and applications

Applied Microbiology and Biotechnology, 2010
Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiological products.
S. Ishii, Kanako Tago, K. Senoo
semanticscholar   +4 more sources

Isolation of diosgenin by microbiological hydrolysis of saponin

Archives of Biochemistry and Biophysics, 1955
Abstract By microbial hydrolysis the saponins in pulverized Dioscorea tuber have been cleaved into the component parts, diosgenin and the sugar moieties. In one case a derivative, Δ 4 -diosgenone, was isolated. Although a wide range of conditions may be used in this conversion, the simplest include a mixed culture fermentation of pulverized fresh ...
John W. Rothrock   +2 more
openaire   +3 more sources

A Simplified Apparatus for the Microbiological Isolation of Calves

British Veterinary Journal, 1976
SUMMARY An isolator has been developed for rearing gnotobiotic and specific pathogen free (SPF) calves. The isolator consisted of relatively cheap components which could be rapidly assembled; the upper half being a flexible polyvinyl chloride (PVC) canopy clamped to a glass fibre tank.
D.C. Davies   +2 more
openaire   +3 more sources

Isolation of Bacillus thuringiensis for Microbiological Control of Insects

Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite Naturwissenschaftliche Abteilung: Mikrobiologie der Landwirtschaft, der Technologie und des Umweltschutzes, 1978
Eleven of 180 local isolates, belonging to Bacillus thuringiensis, were tested for their pathogenicity to pinkbollworm larvae (Pectinophora gossypiella). Three strains, namely RS-25, RS-35, and RS-45, showed a positive insecticidal activity. Their activities based on the crystalline inclusion bodies.
Rasmy M. Attia, Samia A. Ali
openaire   +3 more sources

Spectrum and Susceptibilities of Microbiologic Isolates in the Endophthalmitis Vitrectomy Study

American Journal of Ophthalmology, 1996
To determine the microbiologic spectrum and antibiotic susceptibilities of infecting organisms in postoperative endophthalmitis and to evaluate the effects of operative factors on the microbiologic spectrum.Patients with bacterial endophthalmitis presenting within six weeks of cataract extraction or secondary intraocular lens implantation (IOL) were ...
Michael Barza   +6 more
openaire   +5 more sources

Flexible film isolators: microbiological safety tests

Journal of Hospital Infection, 1983
Flexible film isolators, used in either a positive pressure mode to protect the contents or in a negative pressure mode to protect the environment outside the isolator, have been available for some years. They have been used to protect experimental animals held in germ-free conditions (Trexler, 1971), to house immunosuppressed patients (Trexler, Spiers
B.A. Page-Roberts   +2 more
openaire   +3 more sources

Handbook of Media for Environmental Microbiology

, 2019
The Handbook of Media for Environmental Biology is a compilation of the formulations, methods of preparation, and applications for media used for the isolation and cultivation of microorganisms from environmental sources.
R. Atlas
semanticscholar   +1 more source

Methods in Applied Soil Microbiology and Biochemistry

, 1996
(Chapter Headings): Introduction. Quality Control and Quality Assurance in Applied Soil Microbiology and Biochemistry. Soil Sampling, Handling, Storage, And Analysis. Enrichment, Isolation and Counting of Soil Microorganisms.
K. Alef, P. Nannipieri
semanticscholar   +1 more source

Microbiologic Study of Strains of Mycoplasma gallisepticum Isolated in Argentina [PDF]

open access: possibleAvian Diseases, 1963
Chronic respiratory disease (CRD) in chickens and turkeys in Argentina was first reported in previous articles (2,3), which described two enzootics. In June, July, and August, 1962, three farms registered new outbreaks of this disease, from which isolations of M. gallisepticum were made.
openaire   +2 more sources

Clinical and microbiological evaluation of eight patients with isolated Achromobacter xylosoxidans

Scandinavian Journal of Infectious Diseases, 2012
Achromobacter xylosoxidans causes infections in patients with underlying disorders. The aim of this study was to evaluate Achromobacter xylosoxidans cases. Eight patients were included, 5 infections and 3 colonizations. Three of them survived and 2 died. The predisposing factor was catheter. Infection control measures could prevent infection.
Maras, Gul   +5 more
openaire   +4 more sources

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