Metabolic Flux Analysis—Linking Isotope Labeling and Metabolic Fluxes [PDF]
Metabolites, 2020 Metabolic flux analysis (MFA) is an increasingly important tool to study metabolism quantitatively. Unlike the concentrations of metabolites, the fluxes, which are the rates at which intracellular metabolites interconvert, are not directly measurable ...
Yujue Wang +4 more
doaj +3 more sources
Chemical isotope labeling for quantitative proteomics. [PDF]
Mass Spectrom Rev, 2023 Advancements in liquid chromatography and mass spectrometry over the last decades have led to a significant development in mass spectrometry‐based proteome quantification approaches.
Tian X, Permentier HP, Bischoff R.
europepmc +7 more sources
Pathways of N2O production by marine ammonia-oxidizing archaea determined from dual-isotope labeling. [PDF]
Proc Natl Acad Sci U S A, 2023 Significance Ammonia-oxidizing archaea (AOA) are the major source of marine nitrous oxide (N2O); however, the cellular kinetics and pathways of archaeal N2O production remain unclear.
Wan XS +8 more
europepmc +2 more sources
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics [PDF]
Molecular & Cellular Proteomics, 2002 Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and ...
Shao‐En Ong +6 more
openalex +2 more sources
The topology of metabolic isotope labeling networks [PDF]
BMC Bioinformatics, 2007 Background Metabolic Flux Analysis (MFA) based on isotope labeling experiments (ILEs) is a widely established tool for determining fluxes in metabolic pathways.
Wiechert Wolfgang +2 more
doaj +4 more sources
Hydrogen isotope labeling unravels origin of soil-bound organic contaminant residues in biodegradability testing [PDF]
Nature CommunicationsBiodegradability testing in soil helps to identify safe synthetic organic chemicals but is still obscured by the formation of soil-bound ‘non-extractable’ residues (NERs).
Sophie Lennartz +4 more
doaj +2 more sources
Science Advances, 2021 Metabolic isotope labeling and imaging mass spectrometry delineate early events of precipitating Aβ plaque pathology. β-Amyloid (Aβ) plaque formation is the major pathological hallmark of Alzheimer’s disease (AD) and constitutes a potentially critical ...
W. Michno +12 more
semanticscholar +1 more source
15N Metabolic Labeling Quantification Workflow in Arabidopsis Using Protein Prospector
Frontiers in Plant Science, 2022 Metabolic labeling using stable isotopes is widely used for the relative quantification of proteins in proteomic studies. In plants, metabolic labeling using 15N has great potential, but the associated complexity of data analysis has limited its usage ...
Ruben Shrestha +7 more
doaj +1 more source
Isotope Calculation Gadgets: A Series of Software for Isotope-Tracing Experiments in Garuda Platform
Metabolites, 2022 Isotope tracing is a powerful technique for elucidating intracellular metabolism. Experiments utilizing this technique involve various processes, such as the correction of natural isotopes.
Nobuyuki Okahashi +3 more
doaj +1 more source
ISOTOPICS – ISOTOPIC LABELING FOR DRUG INNOVATION – H2020 [PDF]
Impact, 2017 ISOTOPICS (H2020 - Marie Sklodowska-Curie Actions - Innovative Training Network; grant number 675071) combines researchers and industry in an innovative effort to de-risk drug development, create new tools for medicine and train first-class radiochemists with dual academic-industrial expertise.
Dugave, Christophe, Hinsinger, Karen
openaire +4 more sources