Results 11 to 20 of about 100,704 (351)

Rapid and reliable RNA resonance assignment by combining chemical and enzymatic stable isotope labeling

Journal of Magnetic Resonance Open, 2022
In this work a rapid RNA assignment approach by combining chemical and enzymatic 13C and 15N stable isotope labeling is introduced. We exemplify the assignment strategy for imino N1H1 purine and N3H3 pyrimidine and aromatic C6H6 pyrimidine, C8H8 purine ...
David Klingler, Matthias Huber, Martin Tollinger, Christoph Kreutz   +3 more
doaj   +1 more source

A large-scale LC-MS dataset of murine liver proteome from time course of heavy water metabolic labeling

Scientific Data, 2023
Metabolic stable isotope labeling with heavy water followed by liquid chromatography coupled with mass spectrometry (LC-MS) is a powerful tool for in vivo protein turnover studies.
Henock M. Deberneh, Doaa R. Abdelrahman, Sunil K. Verma, Jennifer J. Linares, Andrew J. Murton, William K. Russell, Muge N. Kuyumcu-Martinez, Benjamin F. Miller, Rovshan G. Sadygov   +8 more
doaj   +1 more source

GPCR structural characterization by NMR spectroscopy in solution

Acta Biochimica et Biophysica Sinica, 2022
In the human proteome, 826 G-protein-coupled receptors (GPCRs) interact with extracellular stimuli to initiate cascades of intracellular signaling. Determining conformational dynamics and intermolecular interactions are key to understand GPCR function as
Yang Lingyun, Liu Dongsheng, Wüthrich Kurt   +2 more
doaj   +1 more source

Envelope: interactive software for modeling and fitting complex isotope distributions

BMC Bioinformatics, 2008
Background An important aspect of proteomic mass spectrometry involves quantifying and interpreting the isotope distributions arising from mixtures of macromolecules with different isotope labeling patterns.
Sykes Michael T, Williamson James R
doaj   +1 more source

Enzymatic incorporation of an isotope-labeled adenine into RNA for the study of conformational dynamics by NMR.

PLoS ONE, 2022
Solution NMR spectroscopy is a well-established tool with unique advantages for structural studies of RNA molecules. However, for large RNA sequences, the NMR resonances often overlap severely.
Hannes Feyrer, Cenk Onur Gurdap, Maja Marušič, Judith Schlagnitweit, Katja Petzold   +4 more
doaj   +1 more source

Nutritional importance of benthic bacteria for deep-sea nematodes from the Arctic ice margin: Results of an isotope tracer experiment [PDF]

, 2010
A
Guilini, Katja, Middelburg, Jack J., Soetaert, Karline, Van Oevelen, Dick, Vanreusel, Ann   +4 more
core   +2 more sources

Elevated Temperature Effects on Protein Turnover Dynamics in Arabidopsis thaliana Seedlings Revealed by ¹⁵N-Stable Isotope Labeling and ProteinTurnover Algorithm. [PDF]

Int J Mol Sci
Fan KT, Xu Y, Hegeman AD.
europepmc   +3 more sources

Research Progress in Radioiodine Labeling Methods of Biomolecules

Journal of Isotopes, 2023
Radiopharmaceuticals derived from targeted molecules labeled with radioiodine have always been the research hotspots in the field of nuclear medicine. Radioiodine labeling also has a clear application demand in pharmacokinetic research.
WANG Yang;LI Zheng;LIU Yuxia;ZHANG Lan;LI Qingnuan
doaj   +1 more source

Stable isotope probing: Technical considerations when resolving ¹⁵N-labeled RNA in gradients [PDF]

, 2010
RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound.
Addison, Sarah Louise, Lloyd-Jones, Gareth, McDonald, Ian R.   +2 more
core   +2 more sources

Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis

Bio-Protocol, 2018
Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be ...
Yuping Cai, Nan Liu, Zheng-Jiang Zhu
doaj   +1 more source