Results 211 to 220 of about 45,524 (260)
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Stable Isotopic Labeling for Proteomics
2011Since the coining of the term Proteome, the field of Proteomics has developed rapidly and come to rely heavily on mass spectrometry, initially for protein identification but recently the use of stable isotopic labels for protein quantitation has grown in importance.
Ashman, Keith +3 more
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Stable Isotope Labeling in Mammals (SILAM)
Cold Spring Harbor Protocols, 2008INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., 2H, 13C, 15N, or 18O).
Daniel B, McClatchy, John R, Yates
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Isotope Labeling in Insect Cells
2012Target proteins in contemporary NMR are becoming increasingly complex. The interest lies on membrane proteins, multi-domain proteins, secreted proteins with secondary modifications etc., which can often only be expressed in eukaryotic expression hosts. Although E. coli is the organism of choice for expression of proteins in isotope labeled form for NMR
Alvar D, Gossert, Wolfgang, Jahnke
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ACS Chemical Neuroscience, 2020
Deuterium metabolic imaging (DMI) is a novel, 3D, magnetic resonance (MR)-based method to map metabolism of deuterated substrates in vivo. The replacement of protons with deuterons could potentially lead to kinetic isotope effects (KIEs) in which metabolic rates of deuterated substrates are reduced due to the presence of a heavier isotope. Knowledge of
Robin A. de Graaf +3 more
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Deuterium metabolic imaging (DMI) is a novel, 3D, magnetic resonance (MR)-based method to map metabolism of deuterated substrates in vivo. The replacement of protons with deuterons could potentially lead to kinetic isotope effects (KIEs) in which metabolic rates of deuterated substrates are reduced due to the presence of a heavier isotope. Knowledge of
Robin A. de Graaf +3 more
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Labeling Monosaccharides With Stable Isotopes
2015Chemical and chemi-enzymic methods are discussed for the preparation of monosaccharide isotopomers that are singly and multiply labeled with (13)C, (2)H, (17/18)O, and (15)N isotopes. The discussion focuses primarily on chemical methods to incorporate stable isotopes into monosaccharides and not on methods to assemble labeled monosaccharides into more ...
Wenhui, Zhang +2 more
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Synthesis of stable isotope‐labelled firocoxib
Journal of Labelled Compounds and Radiopharmaceuticals, 2020Firocoxib (ML‐1,785,713) is a nonsteroidal, potent, and selective COX‐2 inhibitor, approved for the control of pain and inflammation associated with osteoarthritis in dogs and horses, as well as to control postoperative pain and inflammation in dogs.
Bachir Latli +6 more
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LABELLING OF ANTIGENS BY VARIOUS ISOTOPES
Acta Endocrinologica, 1969ABSTRACT Isotopically labelled protein or polypeptide hormones are chemically and/or structurally different from the native material. This may be a major handicap when biological studies or radioimmunoassays are performed. Labelling procedures with 131I, 125I or by introduction of 3H (exchange, acetylation, alanylation) are described, and the ...
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Unsupervised Identification of Isotope-Labeled Peptides
Analytical Chemistry, 2016In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon substrates and intracellular metabolism determine the distribution of isotopes within amino acids.
Joshua E, Goldford, Igor G L, Libourel
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SAIL – stereo-array isotope labeling
Quarterly Reviews of Biophysics, 2009AbstractOptimal stereospecific and regiospecific labeling of proteins with stable isotopes enhances the nuclear magnetic resonance (NMR) method for the determination of the three-dimensional protein structures in solution. Stereo-array isotope labeling (SAIL) offers sharpened lines, spectral simplification without loss of information and the ability to
Masatsune, Kainosho, Peter, Güntert
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Production of Isotopically Labeled KRAS4b
Isotopically labelled proteins are important reagents in structural biology as well as in targeted drug development. The field continues to advance with complex multi-isotope labeling. We have combined our experience in high-level soluble KRAS4b expression with protocols for isotope incorporation, to achieve reliable and efficient approaches for ...Troy, Taylor, William, Gillette
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