Results 41 to 50 of about 1,839,261 (340)

Spatially resolved isotope labeling [PDF]

Nature Methods, 2019
Electron-energy-loss spectroscopy with a scanning transmission electron microscope enables spatially resolved imaging of isotope-labeled molecules.
openaire   +3 more sources

Mechanistic investigation of the formation of H2 from HCOOH with a dinuclear Ru model complex for formate hydrogen lyase

Science and Technology of Advanced Materials, 2017
We report the mechanistic investigation of catalytic H2 evolution from formic acid in water using a formate-bridged dinuclear Ru complex as a formate hydrogen lyase model.
Taisuke Tokunaga, Takeshi Yatabe, Takahiro Matsumoto, Tatsuya Ando, Ki-Seok Yoon, Seiji Ogo   +5 more
doaj   +1 more source

Triple SILAC identified progestin-independent and dependent PRA and PRB interacting partners in breast cancer

Scientific Data, 2021
Measurement(s) progesterone receptor • isoform Technology Type(s) affinity purification method • stable isotope labeling by amino acids in cell culture Factor Type(s) progestin presence • biological replicate Machine-accessible metadata file describing ...
Prangwan Pateetin, Gyorgy Hutvagner, Matthew P. Padula, Eileen M. McGowan, Viroj Boonyaratanakornkit   +4 more
doaj   +1 more source

Determination and Analysis of 16 Mycotoxins in Medicinal and Edible Traditional Chinese Medicine

Shipin gongye ke-ji, 2022
Objective: An analytical method was established for the determination of 16 mycotoxins in traditional Chinese medicines (TCM) by isotope labeling-ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), and 483 medicinal and edible ...
Fenghua LI, Zuohua LI, Li YANG, Yongchun YUAN, Suxiang WANG, Aixiang WANG, Wei LI, Fengjia ZHENG   +7 more
doaj   +1 more source

Recent developments in heterocycle labeling with carbon isotopes [PDF]

Journal of Labelled Compounds and Radiopharmaceuticals, 2018
Heterocycles play an essential role in modern pharmaceutical and agrochemical developments, representing a very common structural unit in marketed drugs. Over the 46 new drugs approved in 2017 by the FDA, 25 contain in their structure a heterocyclic core.
Antonio Del Vecchio, Gianluca Destro, Frédéric Taran, Davide Audisio   +3 more
openaire   +6 more sources

Tonic signaling of the B‐cell antigen‐specific receptor is a common functional hallmark in chronic lymphocytic leukemia cell phosphoproteomes at early disease stages

Molecular Oncology, EarlyView.
B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez, Pablo Juanes‐Velasco, Marina L. García‐Vaquero, Conrad Droste, Alicia Landeira‐Viñuela, Miguel Alcoceba, Helena Fidalgo‐Gómez, Sara Misiego‐Herrero, Almudena Navarro‐Bailón, Mónica Baile, José M. Bastida, Jose Manuel Sanchez‐Santos, Rafael Góngora, Julia Almeida, Marcos Gonzalez‐Diaz, Alberto Orfao, Javier De Las Rivas, Manuel Fuentes   +17 more
wiley   +1 more source

Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands

Journal of Biomolecular NMR, 2019
The physiological role of proteins is frequently linked to interactions with non-protein ligands or posttranslational modifications. Structural characterization of these complexes or modified proteins by NMR may be difficult as the ligands are usually ...
K. Tripsianes, Ulrike Schütz, L. Emmanouilidis, G. Gemmecker, M. Sattler   +4 more
semanticscholar   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Pyridine-based strategies towards nitrogen isotope exchange and multiple isotope incorporation

Nature Communications
Isotopic labeling is at the core of health and life science applications such as nuclear imaging, metabolomics and plays a central role in drug development. The rapid access to isotopically labeled organic molecules is a sine qua non condition to support
Minghao Feng, Maylis Norlöff, Benoit Guichard, Steven Kealey, Timothée D’Anfray, Pierre Thuéry, Frédéric Taran, Antony Gee, Sophie Feuillastre, Davide Audisio   +9 more
doaj   +1 more source

Rapid and reliable RNA resonance assignment by combining chemical and enzymatic stable isotope labeling

Journal of Magnetic Resonance Open, 2022
In this work a rapid RNA assignment approach by combining chemical and enzymatic 13C and 15N stable isotope labeling is introduced. We exemplify the assignment strategy for imino N1H1 purine and N3H3 pyrimidine and aromatic C6H6 pyrimidine, C8H8 purine ...
David Klingler, Matthias Huber, Martin Tollinger, Christoph Kreutz   +3 more
doaj