Results 41 to 50 of about 100,704 (351)
Isotope Labeling in Insect Cells [PDF]
, 2011 Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-
Krishna, Saxena +3 more
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Science and Technology of Advanced Materials, 2017 We report the mechanistic investigation of catalytic H2 evolution from formic acid in water using a formate-bridged dinuclear Ru complex as a formate hydrogen lyase model.
Taisuke Tokunaga +5 more
doaj +1 more source
, 2014 Citation: 'isotopic labelling' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.I03345 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
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The topology of metabolic isotope labeling networks
BMC Bioinformatics, 2007 Background Metabolic Flux Analysis (MFA) based on isotope labeling experiments (ILEs) is a widely established tool for determining fluxes in metabolic pathways.
Wiechert Wolfgang +2 more
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One‐shot 13C15N‐metabolic flux analysis for simultaneous quantification of carbon and nitrogen flux
Molecular Systems Biology, 2023 Metabolic flux is the final output of cellular regulation and has been extensively studied for carbon but much less is known about nitrogen, which is another important building block for living organisms.
Khushboo Borah Slater +10 more
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O−O Bond Formation and Liberation of Dioxygen Mediated by N5‐Coordinate Non‐Heme Iron(IV) Complexes [PDF]
, 2019 Formation of the O−O bond is considered the critical step in oxidative water cleavage to produce dioxygen. High‐valent metal complexes with terminal oxo (oxido) ligands are commonly regarded as instrumental for oxygen evolution, but direct experimental ...
Diekmann, Marek +9 more
core +1 more source
Synthesis of isotopically labelled ubiquinones
Tetrahedron Letters, 1992 Abstract Ubiquinones ( 1 ) of various chain lengths have been synthesized. The key step involves the condensation of a long chain polyunsaturated alcohol onto an aromatic intermediate. The synthetic methodology allows for the incorporation of isotopic labels in the methoxy groups attached to the ring portion of the molecule.
Duralski, A, Watts, A
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Chemoenzymatic Assembly of Isotopically Labeled Folates [PDF]
Journal of the American Chemical Society, 2017 Pterin-containing natural products have diverse functions in life, but an efficient and easy scheme for their in vitro synthesis is not available. Here we report a chemoenzymatic 14-step, one-pot synthesis that can be used to generate 13C- and 15N-labeled dihydrofolates (H2F) from glucose, guanine, and p-aminobenzoyl-l-glutamic acid.
Antonio Angelastro +4 more
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FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source