Results 51 to 60 of about 92,950 (293)
One‐shot 13C15N‐metabolic flux analysis for simultaneous quantification of carbon and nitrogen flux
Molecular Systems Biology, 2023 Metabolic flux is the final output of cellular regulation and has been extensively studied for carbon but much less is known about nitrogen, which is another important building block for living organisms.
Khushboo Borah Slater +10 more
doaj +1 more source
Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes
FEBS Open Bio, EarlyView.We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde +3 more
wiley +1 more source
Characterization of Cell Glycocalyx with Mass Spectrometry Methods. [PDF]
, 2019 The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function.
Lebrilla, Carlito B +3 more
core +2 more sources
Biomolecules, 2020 Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm ...
Hirokazu Yagi +9 more
doaj +1 more source
Discovery and Targeted Proteomic Studies Reveal Striatal Markers Validated for Huntington's Disease
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Clinical trials for Huntington's disease (HD) enrolling persons before clinical motor diagnosis (CMD) lack validated biomarkers. This study aimed to conduct an unbiased discovery analysis and a targeted examination of proteomic biomarkers scrutinized by clinical validation. Methods Cerebrospinal fluid was obtained from PREDICT‐HD and
Daniel Chelsky +8 more
wiley +1 more source
Unveiling the metabolic fate of monosaccharides in cell membranes with glycomic and glycoproteomic analyses. [PDF]
, 2019 Cell membrane protein glycosylation is dependent on the metabolic state of the cell as well as exogenous nutrients available. Although the metabolism and interconversion of monosaccharides have been well-studied, their incorporation into cell surface ...
Cheng, Zhi +5 more
core
SIMPEL: using stable isotopes to elucidate dynamics of context specific metabolism
Communications BiologyThe capacity to leverage high resolution mass spectrometry (HRMS) with transient isotope labeling experiments is an untapped opportunity to derive insights on context-specific metabolism, that is difficult to assess quantitatively.
Shrikaar Kambhampati +7 more
doaj +1 more source
Scientific Data, 2021 Measurement(s) progesterone receptor • isoform Technology Type(s) affinity purification method • stable isotope labeling by amino acids in cell culture Factor Type(s) progestin presence • biological replicate Machine-accessible metadata file describing ...
Prangwan Pateetin +4 more
doaj +1 more source
CSF Monoamine Metabolites and Cognitive Trajectory in Early Parkinson's Disease
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Background Imaging and postmortem studies indicate that abnormalities in monoaminergic neurotransmission contribute to cognitive impairment in Parkinson's disease (PD). However, it remains uncertain if cerebrospinal fluid (CSF) monoamine metabolites can serve as biomarkers of cognitive decline in early PD.
Jing‐Yu Shao +7 more
wiley +1 more source
Isotope Labeling in Mammalian Cells [PDF]
, 2011 Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression.
Arpana, Dutta +3 more
openaire +2 more sources