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Production of Isotopically Labeled KRAS4b

Isotopically labelled proteins are important reagents in structural biology as well as in targeted drug development. The field continues to advance with complex multi-isotope labeling. We have combined our experience in high-level soluble KRAS4b expression with protocols for isotope incorporation, to achieve reliable and efficient approaches for ...
Troy, Taylor, William, Gillette
openaire   +2 more sources

Unsupervised Identification of Isotope-Labeled Peptides

Analytical Chemistry, 2016
In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon substrates and intracellular metabolism determine the distribution of isotopes within amino acids.
Joshua E, Goldford, Igor G L, Libourel
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Isotopic labelings for mechanistic studies

The intricate mechanisms in the biosynthesis of terpenes belong to the most challenging problems in natural product chemistry. Methods to address these problems include the structure-based site-directed mutagenesis of terpene synthases, computational approaches, and isotopic labeling experiments. The latter approach has a long tradition in biosynthesis
Houchao, Xu, Jeroen S, Dickschat
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Mapping isotopically labeled alanine

Science, 2019
Electron Microscopy Electron microscopy of organic materials must avoid the destructive effects of electron beam impact. One approach is to measure vibrational spectra with electron energy-loss spectroscopy in a mode where the electron beam grazes the sample and couples to it through evanescent modes. Hachtel et al. used such methods to probe carbon-12–
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Radiopharmaceuticals labelled with bromine isotopes

International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes, 1986
B, Maziere, C, Loc'h
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Isotopic labelling of proanthocyanidins

1997
International ...
Deprez, S.   +3 more
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Isotopically labelled quinones

2010
Mieczysaw Zieliski, Marianna Kaska
openaire   +1 more source

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