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Production of Isotopically Labeled KRAS4b
Isotopically labelled proteins are important reagents in structural biology as well as in targeted drug development. The field continues to advance with complex multi-isotope labeling. We have combined our experience in high-level soluble KRAS4b expression with protocols for isotope incorporation, to achieve reliable and efficient approaches for ...Troy, Taylor, William, Gillette
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Unsupervised Identification of Isotope-Labeled Peptides
Analytical Chemistry, 2016In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon substrates and intracellular metabolism determine the distribution of isotopes within amino acids.
Joshua E, Goldford, Igor G L, Libourel
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Isotopic labelings for mechanistic studies
The intricate mechanisms in the biosynthesis of terpenes belong to the most challenging problems in natural product chemistry. Methods to address these problems include the structure-based site-directed mutagenesis of terpene synthases, computational approaches, and isotopic labeling experiments. The latter approach has a long tradition in biosynthesisHouchao, Xu, Jeroen S, Dickschat
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Mapping isotopically labeled alanine
Science, 2019Electron Microscopy Electron microscopy of organic materials must avoid the destructive effects of electron beam impact. One approach is to measure vibrational spectra with electron energy-loss spectroscopy in a mode where the electron beam grazes the sample and couples to it through evanescent modes. Hachtel et al. used such methods to probe carbon-12–
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Radiopharmaceuticals labelled with bromine isotopes
International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes, 1986B, Maziere, C, Loc'h
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Isotopic labelling of proanthocyanidins
1997International ...
Deprez, S. +3 more
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