Results 51 to 60 of about 54,813 (313)

Synthesis of isotopically labelled ubiquinones

Tetrahedron Letters, 1992
Abstract Ubiquinones ( 1 ) of various chain lengths have been synthesized. The key step involves the condensation of a long chain polyunsaturated alcohol onto an aromatic intermediate. The synthetic methodology allows for the incorporation of isotopic labels in the methoxy groups attached to the ring portion of the molecule.
Duralski, A, Watts, A
openaire   +2 more sources

Chemoenzymatic Assembly of Isotopically Labeled Folates [PDF]

Journal of the American Chemical Society, 2017
Pterin-containing natural products have diverse functions in life, but an efficient and easy scheme for their in vitro synthesis is not available. Here we report a chemoenzymatic 14-step, one-pot synthesis that can be used to generate 13C- and 15N-labeled dihydrofolates (H2F) from glucose, guanine, and p-aminobenzoyl-l-glutamic acid.
Antonio Angelastro, William M. Dawson, Louis Y. P. Luk, E. Joel Loveridge, Rudolf K. Allemann   +4 more
openaire   +6 more sources

Homological Region Adjacency Tree for a 3D Binary Digital Image via HSF Model [PDF]

, 2019
Given a 3D binary digital image I, we define and compute an edge-weighted tree, called Homological Region Tree (or Hom-Tree, for short). It coincides, as unweighted graph, with the classical Region Adjacency Tree of black 6-connected components (CCs ...
Blanco Trejo, Sergio, Díaz del Río, Fernando, Molina Abril, Helena, Real Jurado, Pedro   +3 more
core   +1 more source

Virtual shadow modules and their link invariants

, 2012
We introduce an algebra Z[X,S] associated to a pair (X,S) of a virtual birack X and X-shadow S. We use modules over Z[X,S] to define enhancements of the virtual birack shadow counting invariant, extending the birack shadow module invariants to virtual ...
Blankstein, Jackson, Kim, Susan, Lepel, Catherine, Nelson, Sam, Sanderson, Nicole   +4 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions

Nature Communications, 2018
Measuring precise protein turnover rates in animals is technically challenging at the proteomic level. Here, Fornasiero and colleagues use isotopic labeling with mass spectrometry and mathematical modeling to accurately determine protein lifetimes in the
Eugenio F. Fornasiero, Sunit Mandad, Hanna Wildhagen, Mihai Alevra, Burkhard Rammner, Sarva Keihani, Felipe Opazo, Inga Urban, Till Ischebeck, M. Sadman Sakib, Maryam K. Fard, Koray Kirli, Tonatiuh Pena Centeno, Ramon O. Vidal, Raza-Ur Rahman, Eva Benito, André Fischer, Sven Dennerlein, Peter Rehling, Ivo Feussner, Stefan Bonn, Mikael Simons, Henning Urlaub, Silvio O. Rizzoli   +23 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Isotopic Analysis of Sporocarp Protein and Structural Material Improves Resolution of Fungal Carbon Sources [PDF]

, 2016
Fungal acquisition of resources is difficult to assess in the field. To determine whether fungi received carbon from recent plant photosynthate, litter or soil-derived organic (C:N bonded) nitrogen, we examined differences in δ13C among bulk tissue ...
Chen, Janet, Hobbie, Erik A., Hofmockel, Kirsten S.   +2 more
core   +3 more sources

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins

Molecules, 2021
An efficient self-cleavable purification tag could be a powerful tool for purifying recombinant proteins and peptides without additional proteolytic processes using specific proteases.
A. Sesilja Aranko, Hideo Iwaï
doaj   +1 more source