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Isozymes of Phenylalanine Hydroxylase [PDF]
Science, 1972 Three isozymes of phenylalanine hydroxylase exist in adult rat liver. They are chromatographically unique. Partial characterization suggests that they are similar in chemical properties and differ only in charge. Estimation of the Stokes radii indicates that the isozymes have similar molecular weights of about 200,000. Two isozymes exist in human fetal
Paul J. Geiger +3 more
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Science, 1966
A new method of starch-gel electrophoresis using a technique of staining dependent on enzyme activity has been employed to demonstrate the isozymes of aldolase from a variety of human, rat, and frog tissues. Five of these isozymes were detected in man, seven in the rat, and at least four in the frog. The abilities of these isozymes to cleave fructose-1,
Jose M. Trujillo +2 more
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A new method of starch-gel electrophoresis using a technique of staining dependent on enzyme activity has been employed to demonstrate the isozymes of aldolase from a variety of human, rat, and frog tissues. Five of these isozymes were detected in man, seven in the rat, and at least four in the frog. The abilities of these isozymes to cleave fructose-1,
Jose M. Trujillo +2 more
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Anchoring proteins for protein kinase C: a means for isozyme selectivity.
The FASEB Journal, 1998Protein kinase C (PKC) isozymes comprise a family of related enzymes. There are only limited differences between these isozymes in substrate specificity or sensitivity to activators.
D. Mochly‐Rosen, A. Gordon
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Malic Dehydrogenase Isozymes [PDF]
Nature, 1965 IT has been shown previously1–3 that at least two isozymes of malic dehydrogenase are present in the extracts of various tissues. In addition, the distinct characteristics of these isozymes have been demonstrated by the recent reports4–6.
Kozo Yamada +2 more
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The heme oxygenase system: a regulator of second messenger gases.
Annual Review of Pharmacology and Toxicology, 1997The heme oxygenase (HO) system consists of two forms identified to date: the oxidative stress-inducible protein HO-1 (HSP32) and the constitutive isozyme HO-2. These proteins, which are different gene products, have little in common in primary structure,
M. Maines
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Cyclooxygenases: structural, cellular, and molecular biology.
Annual Review of Biochemistry, 2000The prostaglandin endoperoxide H synthases-1 and 2 (PGHS-1 and PGHS-2; also cyclooxygenases-1 and 2, COX-1 and COX-2) catalyze the committed step in prostaglandin synthesis.
W. Smith, D. Dewitt, R. Garavito
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Lipoxygenase isozymes of peanut
Lipids, 1975AbstractLipoxygenase was isolated and partially purified from peanut seed by ammonium sulfate precipitation, gel filtration, and ion exchange column chromatography. Three isozymes of lipoxygenase were identified. Two had pH optima of 6.2, and the other an optimum of 8.3. Molecular weight of each isozyme was 7.3×104, as determined by gel filtration. The
Harold E. Pattee +2 more
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BioScience, 1975
Isozymes are now a common part of the scientific vocabulary, but their recognition is relatively recent, having been first announced in 1959 (Markert and Mqller). Prior to that time, molecular heterogeneity had often been noted in enzyme preparations, but such heterogeneity was usually attributed to contaminants or to partially denatured or degraded ...
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Isozymes are now a common part of the scientific vocabulary, but their recognition is relatively recent, having been first announced in 1959 (Markert and Mqller). Prior to that time, molecular heterogeneity had often been noted in enzyme preparations, but such heterogeneity was usually attributed to contaminants or to partially denatured or degraded ...
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Microheterogeneity of Kasahara isozyme
Clinica Chimica Acta, 1986The microheterogeneity of Kasahara isozyme was investigated by affinity electrophoresis with Con A as the affinity ligand in combination with polyacrylamide gradient gel electrophoresis. On two-dimensional Con A-containing agarose gel electrophoresis, the Kasahara isozyme was separated into three molecular species.
Kazuhisa Taketa +8 more
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Urological Research, 1990
We determined concentrations of alpha and gamma-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of alpha-enolase were 4,170 +/- 2,040 ng/mg protein in normal testis (n = 8) and 8,140 +/- 4,480 ng/mg protein in seminoma (n = 8).
Tatsuya Nagai +4 more
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We determined concentrations of alpha and gamma-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of alpha-enolase were 4,170 +/- 2,040 ng/mg protein in normal testis (n = 8) and 8,140 +/- 4,480 ng/mg protein in seminoma (n = 8).
Tatsuya Nagai +4 more
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