African dung beetle Onthophagus gazella Fabricius (Coleoptera: Scarabaeidae) esterase isozymes
African beetles Onthophagus gazella from both sexes were analyzed by electrophoresis for an investigation of esterase isozymes using alpha-naphthyl propionate and methylumbelliferyl propionate as substrates. Only one of the esterases (Est.
E. MARTINS, E. P. B. CONTEL
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X-chromosome inactivation mosaicism in the three germ layers and the germ line of the mouse embryo [PDF]
Electrophoretic variant forms of the X-linked enzyme phosphoglycerate kinase (PGK-1, E.C.2, 7, 2, 3) have been used to examine X-chromosome mosaicism in tissues from 121/2-day post coitum heterozygous female mouse embryos.
Fosten, M., McMahon, A., Monk, M.
core
DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]
We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M+7 more
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ABSTRACT Cdt1 is a mixed folded protein critical for DNA replication licensing and it also has a “moonlighting” role at the kinetochore via direct binding to microtubules and the Ndc80 complex. However, it is unknown how the structure and conformations of Cdt1 could allow it to participate in these multiple, unique sets of protein complexes.
Kyle P. Smith+9 more
wiley +1 more source
How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?
Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez;
Kwiatek Michał+6 more
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Distinct forebrain and cerebellar isozymes of type II Ca^(2+)/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction [PDF]
Forebrain and cerebellar Type II Ca2+/calmodulin-dependent protein kinases have different subunit compositions. The forebrain holoenzyme, characterized in our laboratory, is a 650-kDa holoenzyme composed of 50-kDa alpha-subunits and 60-kDa beta-subunits ...
Kennedy, Mary B., Miller, Stephen G.
core
Computational Studies of Enzymes for C−F Bond Degradation and Functionalization
Enzyme‐mediated strategies for C−F bond cleavage offer a promising solution for addressing the environmental challenges posed by the Forever Chemicals. This review discusses how computational approaches – including molecular docking, molecular dynamics, QM/MM, and bioinformatics – enhance the understanding of enzymatic degradation mechanisms, guiding ...
Kendra M. Cunningham+2 more
wiley +1 more source
Variability among inbred lines and RFLP mapping of sunflower isozymes
Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and ...
Carrera Alicia D.+5 more
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Conducting clinical studies on drug–drug‐gene interactions (DDGIs) and extrapolating the findings into clinical dose recommendations is challenging due to the high complexity of these interactions. Here, physiologically‐based pharmacokinetic (PBPK) modeling networks present a new avenue for exploring such complex scenarios, potentially informing ...
Simeon Rüdesheim+14 more
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Polimorfismo isoenzimático en cuatro razas y un híbrido de Bactris gasipaes (Palmae)
Se estandarizó un sistema de electroforesis de isoenzimas con tejido de hojas que permitió la identificación del polimorfismo fenético entre cuatro razas y un híbrido de pejibaye Bactris gasipaes provenientes de Brasil, Perú, Bolivia, Panamá y Costa Rica.
Sonia Rojas-Vargas+2 more
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