Results 61 to 70 of about 376,522 (308)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

DFIT: An R Package for Raju's Differential Functioning of Items and Tests Framework

open access: yesJournal of Statistical Software, 2017
This paper presents DFIT, an R package that implements the differential functioning of items and tests framework as well as the Monte Carlo item parameter replication approach for producing cut-off points for differential item functioning indices ...
Víctor H. Cervantes
doaj   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

open access: yesFEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino   +7 more
wiley   +1 more source

Linking Error Estimation in Fixed Item Parameter Calibration: Theory and Application in Large-Scale Assessment Studies

open access: yesFoundations
In fixed item parameter calibration (FIPC), an item response theory (IRT) model is estimated with item parameters fixed at reference values to estimate the distribution parameters within a specific group.
Alexander Robitzsch
doaj   +1 more source

An Evaluation of Overall Goodness-of-Fit Tests for the Rasch Model

open access: yesFrontiers in Psychology, 2019
For assessing the fit of item response theory models, it has been suggested to apply overall goodness-of-fit tests as well as tests for individual items and item pairs.
Rudolf Debelak
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

Item Response Theory for trait assessment in randomized item pool for computer based test

open access: yesScientific African
The rapid adoption of computer-based examinations (CBE) has raised concerns regarding fairness and reliability in assessing student performance. This study examines randomized test item fairness to test takers’ traits in CBE through the application of ...
Rhydal Esi Eghan   +5 more
doaj   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

open access: yesFEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo   +2 more
wiley   +1 more source

BAYES MODAL ESTIMATION IN ITEM RESPONSE MODELS* [PDF]

open access: yesETS Research Report Series, 1985
ABSTRACTThis article describes a Bayesian framework for estimation in item response models, with two‐stage prior distributions on both item and examinee populations. Strategies for point and interval estimation are discussed, and a general procedure based on the EM algorithm is presented. Details are given for implementation under one‐, two‐, and three‐
openaire   +2 more sources

Home - About - Disclaimer - Privacy