Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Flexible Item Response Models for Count Data: The Count Thresholds Model. [PDF]
Appl Psychol Meas, 2022 Tutz G.
europepmc +1 more source
Flexible Rasch Mixture Models with Package psychomix [PDF]
Measurement invariance is an important assumption in the Rasch model and mixture models constitute a flexible way of checking for a violation of this assumption by detecting unobserved heterogeneity in item response data.
Carolin Strobl +3 more
core
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
The Impact and Detection of Uniform Differential Item Functioning for Continuous Item Response Models. [PDF]
Educ Psychol Meas, 2023 Finch WH.
europepmc +1 more source
Benchmarking by Item Response Theory (BIRTH): método de benchmarking utilizando a teoria da resposta ao item na construção de escalas de competitividade [PDF]
, 2014 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Produção, Florianópolis, 2014.Este trabalho desenvolve um novo método de benchmarking, que permite a construção de escalas de ...
Pacheco, Juliano Anderson
core
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
plink: An R Package for Linking Mixed-Format Tests Using IRT-Based Methods [PDF]
The R package plink has been developed to facilitate the linking of mixed-format tests for multiple groups under a common item design using unidimensional and multidimensional IRT-based methods.
Jonathan P. Weeks
core
Using the area between two item response functions to index differential item functioning : a generalized approach [PDF]
, 1994 The area between two item response functions was used to construct two indicators of differential item functioning. The first indicator was designed from the integral of the difference of the two response functions across six standard deviations of ...
Penny, James A. +1 more
core
Translophagy—A potential link between autophagy impairment and translational errors
FEBS Letters, EarlyView.Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk +11 more
wiley +1 more source