Results 101 to 110 of about 25,211 (209)

MARQUIS: A multiplex method for absolute quantification of peptides and posttranslational modifications [PDF]

, 2014
Absolute quantification of protein expression and posttranslational modifications by mass spectrometry has been challenging due to a variety of factors, including the potentially large dynamic range of phosphorylation response.
Curran, Timothy G., Ma, Daniel J., Sarkaria, Jann N., White, Forest M., Zhang, Yi   +4 more
core   +2 more sources

Exploring microorganism–host interactions: Emerging organoid models and analytical approaches

mLife, Volume 5, Issue 1, Page 17-34, February 2026.
Abstract Microorganisms play a vital role in human health through their interactions with the body. Studies of host–microbe mechanisms and interactions are crucial for advancing health management. Recently, the organoid‐based models have provided new platforms in this field.
Yue Shi, Min Xu, Yanhong Huang, Jing Qu, Shumin Liao, Yingzi Liu, Liang Li   +6 more
wiley   +1 more source

Comparison of iTRAQ and SWATH in a clinical study with multiple time points

Clinical Proteomics, 2018
Background Advances in mass spectrometry have accelerated biomarker discovery in many areas of medicine. The purpose of this study was to compare two mass spectrometry (MS) methods, isobaric tags for relative and absolute quantitation (iTRAQ) and ...
Antti Jylhä, Janika Nättinen, Ulla Aapola, Alexandra Mikhailova, Matti Nykter, Lei Zhou, Roger Beuerman, Hannu Uusitalo   +7 more
doaj   +1 more source

Toward quantitative phosphotyrosine profiling in vivo [PDF]

, 2012
Tyrosine phosphorylation is a dynamic reversible post-translational modification that regulates many aspects of cell biology. To understand how this modification controls biological function, it is necessary to not only identify the specific sites of ...
Johnson, Hannah, White, Forest M.
core   +1 more source

Advancing Extracellular Vesicle Research: A Review of Systems Biology and Multiomics Perspectives

PROTEOMICS, Volume 26, Issue 2-3, Page 33-80, February 2026.
ABSTRACT Extracellular vesicles (EVs) are membrane‐bound vesicles secreted by various cell types into the extracellular space and play a role in intercellular communication. Their molecular cargo varies depending on the cell of origin and its functional state.
Gloria Kemunto, Samaneh Ghadami, Kristen Dellinger   +2 more
wiley   +1 more source

Quantitative proteomics of a B12 -dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism [PDF]

, 2017
The unicellular green alga Lobomonas rostrata requires an external supply of vitamin B12 (cobalamin) for growth, which it can obtain in stable laboratory cultures from the soil bacterium Mesorhizobium loti in exchange for photosynthate.
Amin, Amin, Arrigo, Ashen, Beliaev, Bertrand, Bertrand, Biller, Bradford, Catalano, Chauvin, Cooper, Copley, Croft, Durham, Eisenreich, Elias, Emerich, Falkowski, Fernandes, Geng, González, Grant, Helliwell, Helliwell, Helliwell, Hold, Hom, Kanehisa, Kazamia, Kazamia, Koch, Koch, Liu, Lodwig, Lohse, Longworth, Matsuo, Maxwell, Mus, Nedelcu, Noirel, Ohlrogge, Paerl, Palmer, Pandhal, Panter, Park, Pfaffl, Saalbach, Saibil, Schmollinger, Shargool, Sher, Song, Tai, Thiel, Untergasser, Vizcaíno, Wagner-Döbler, Wienkoop, Xie, Zhang   +62 more
core   +2 more sources

Preparation of Peptides from Yeast Cells for iTRAQ Analysis [PDF]

Cold Spring Harbor Protocols, 2011
INTRODUCTIONA commercial reagent, known as the isobaric tag for relative and absolute quantification (iTRAQ), makes it possible to analyze multiple samples simultaneously. The ability of iTRAQ to compare relative protein abundances across as many as eight samples is a significant advantage over other stable isotope strategies, such as stable isotope ...
openaire   +2 more sources

Quantitative definition and monitoring of the host cell protein proteome using iTRAQ: a study of an industrial mAb producing CHO-S cell line [PDF]

, 2016
There are few studies defining CHO host cell proteins (HCPs) and the flux of these throughout a downstream purification process. Here we have applied quantitative iTRAQ proteomics to follow the HCP profile of an antibody (mAb) producing CHO-S cell line ...
Aboulaich, Bailey-Kellogg, Beatson, Bee, Bracewell, Clincke, Doneanu, Gagnon, Gao, Grzeskowiak, Guiochon, Hayes, Hogwood, Hogwood, Hogwood, Huang, Jin, Josse, Joucla, Kao, Koterba, Levy, Liu, Mead, Ow, Ow, Pezzini, Reisinger, Robert, Schenauer, Shukla, Shukla, Sisodiya, Tait, Tarrant, Thompson, Tscheliessnig, Valente, Valente, Walsh, Wang, Yuk, Zhang, Zhu-Shimoni   +43 more
core   +2 more sources

Hepatic proteome network data in zebrafish (Danio rerio) liver following dieldrin exposure

Data in Brief, 2019
Dieldrin is an environmental contaminant that adversely affects aquatic organisms. The data presented in this study are proteomic data collected in liver of zebrafish that were exposed to the pesticide in a dietary exposure.
Denina B.D. Simmons, Andrew M. Cowie, Jin Koh, James P. Sherry, Christopher J. Martyniuk   +4 more
doaj   +1 more source

Analysis of the hippocampal proteome in ME7 prion disease reveals a predominant astrocytic signature and highlights the brain-restricted production of clusterin in chronic neurodegeneration

, 2013
Prion diseases are characterized by accumulation of misfolded protein, gliosis, synaptic dysfunction, and ultimately neuronal loss. This sequence, mirroring key features of Alzheimer disease, is modeled well in ME7 prion disease.
Asuni, Ayodeji A, Bailey, Joanne, Gray, Bryony, O'Connor, Vincent, Perry, V Hugh, Skipp, Paul   +5 more
core   +1 more source