Results 101 to 110 of about 1,125,930 (294)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Dynamic flexible job shop co-scheduling optimization based on graph neural network and deep reinforcement learning

Operations Research Perspectives
With the continuous advancement of manufacturing, job shop scheduling faces complex dynamic disturbances that pose significant challenges to production.
Lan Yang, Zhong Yang, Li Bi, Xiaogang Jiao   +3 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A control strategy for promoting shop-floor stability [PDF]

This research aimed to study real-time shop floor control problem in a manufacturing environment with dual resource (machine and labour), under impact of machine breakdowns.
Huang, You Sen
core  

JOB-monitor 2020

, 2020
De JOB-monitor is in 2001 door Jongeren Organisatie Beroepsonderwijs (JOB) opgezet om de tevredenheid van mbo-studenten te meten. Elke twee jaar laat JOB de monitor uitvoeren door onderzoeksbureau ResearchNed.
ResearchNed
core   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Un algoritmo genético para el problema de Job Shop Flexible A genetic algorithm for the Flexible Job Shop problem

Ingeniare: Revista Chilena de Ingeniería, 2011
En este estudio se propone e implementa computacionalmente un algoritmo genético secuencial para resolver el problema del Job Shop Flexible (existente en la Gestión de Operaciones), el cual es parte de la familia de los problemas de programación de ...
Rosa Medina Durán, Lorena Pradenas Rojas, Víctor Parada Daza   +2 more
doaj  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Transgenic Algorithm Applied to the Job Shop Rescheduling Problem

Ingeniería
Context: Job sequencing has been approached from a static perspective, without considering the occurrence of unexpected events that might require modifying the schedule, thereby affecting its performance measures.
Néstor Andrés Beltrán-Bernal, José Ignacio Rodríguez-Molano, Diego Ernesto Mendoza-Patiño   +2 more
doaj   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source