Results 81 to 90 of about 1,132,012 (246)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Solving Flexible Job-Shop Scheduling Problem Using Gravitational Search Algorithm and Colored Petri Net

Journal of Applied Mathematics, 2012
Scheduled production system leads to avoiding stock accumulations, losses reduction, decreasing or even eliminating idol machines, and effort to better benefitting from machines for on time responding customer orders and supplying requested materials in ...
Behnam Barzegar, Homayun Motameni, Hossein Bozorgi   +2 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Proportionate Flow Shop Games [PDF]

In a proportionate flow shop problem several jobs have to be processed through a fixed sequence of machines and the processing time of each job is equal on all machines.By identifying jobs with agents, whose costs linearly depend on the completion time ...
Borm, P.E.M., Estevez Fernandez, M.A., Mosquera, M.A., Hamers, H.J.M.   +3 more
core  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Optimised search heuristic combining valid inequalities and tabu search [PDF]

This paper presents an Optimised Search Heuristic that combines a tabu search method with the verification of violated valid inequalities. The solution delivered by the tabu search is partially destroyed by a randomised greedy procedure, and then the ...
Helena Ramalhinho-Lourenço, Susana Fernandes   +1 more
core  

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Optimization in Job Shop Scheduling Using Alternative Routes

, 2003
The ability of a production system to complete orders on time is a critical measure of customer service. While there is typically a preferred routing for a job through the processing machines, often an alternative route is available that can be used to ...
Davenport, Catherine Elizabeth
core  

Stability of Predictive Control in Job Shop System with Reconfigurable Machine Tools for Capacity Adjustment

Logistics Research, 2019
Due to changes in individual demand, manufacturing processes have become more complex and dynamic. To cope with respective fluctuations as well as machine breakdowns, capacity adjustment is one of the major effective measures.
Qiang Zhang, Michael Freitag, Jürgen Pannek   +2 more
doaj   +1 more source