Results 51 to 60 of about 193 (134)
Ultrasound in Obstetrics &Gynecology, Volume 66, Issue 6, Page 738-746, December 2025.ABSTRACT Objective To evaluate the performance of non‐invasive prenatal testing (NIPT) in vanishing‐twin and multiple pregnancies. Methods This study was conducted as part of the TRIDENT‐2 study, in which NIPT was offered as a first‐tier screening test to women with a multiple pregnancy or vanishing‐twin pregnancy between 1 June 2020 and 31 March 2023 ...
J. C. A. van Eekhout +86 more
wiley +1 more source
半边旗二萜类化合物5F对人翼状胬肉成纤维细胞结缔组织生长因子表达和胶原合成的影响 [PDF]
, 2006 [目的]观察半边旗二萜类化合物5F（5F）对体外培养的人翼状胬肉成纤维细胞（HPF）增殖和胶原合成及结缔组织生长因子（CTGF）表达的影响.[方法]利用手术切除的人翼状胬肉组织进行原代细胞培养,即得到HPF.将不同质量不同浓度（0、8、32、128）μg/mL的5F作用于HPF 24 h,通过噻唑蓝比色法（MTT）检测5F对细胞增殖的作用;用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹（Western blot）检测5F对细胞中CTGFmRNA及蛋白表达水平的影响 ...
何惠娟 +6 more
core
布托啡诺逆转ABCB1 蛋白诱导的白血病多重耐药的 效果和机制
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】研究布托啡诺在临床使用剂量范围内通过抑制ABCB1的转运功能增加白血病耐药细胞的化 疗药物敏感性。【方法】采用HL60及其ABCB1过表达的耐药细胞株HL60/VCR、K562及其ABCB1过表达的耐药 细胞株K562/ADR 均在培养环境中加入含胎牛血清的DMEM 或RPMI-1640的培养液中进行培养。采用MTT法 检测布托啡诺的细胞毒性，通过流式细胞仪检测阿霉素（DOX）在HL60/VCR细胞内的累积程度；加入布托啡诺 培养72 ...
邹志聪 +4 more
doaj
Effects of oyster bioactive peptides BPO-1 on the morphology and ultrastructure of human gastric adenocarcinoma cell line BGC-823 [PDF]
, 2007 目的观察BPO-1对人胃腺癌BGC-823细胞形态与超微结构的影响,以探索BPO-1对胃癌细胞的作用机制。方法采用酸抽提、凝胶柱层析等方法,从牡蛎体内分离提取到牡蛎天然活性多肽组分BPO-1,以细胞凋亡诱导物姜黄素和癌细胞分化诱导物HMBA为平行对照,用光镜、透射电镜观察BPO-1处理细胞的形态和超微结构变化。结果经处理后的BGC-823细胞体积缩小、染色质凝聚、线粒体空泡化、内质网腔扩大和出现凋亡小体等多种典型的细胞凋亡形态和超微结构特征。结论牡蛎天然活性肽BPO-1对人胃腺癌BGC ...
李祺福, 李鹏, 梁盈, 石松林
core
Effect of Phosphoinositide 3-kinase/Protein Kinase B Signaling Pathway on Cell Apoptosis during Chilled Storage of Tan Sheep Meat [PDF]
The hindleg meat of Tan sheep was injected postmortem with the phosphoinositide 3-kinase (PI3K) inhibitor LY294002 at a concentration of 10 μmol/L and stored at 4 ℃.
ZHANG Qian, LUO Ruiming, CHEN Xueyan, LI Rong, WANG Jinxia, HU Lijun
core +1 more source
Research on histone deacetylase inhibitor SAHA-induced apoptosis of multiple myeloma cell line U266 cells and its possible mechanism [PDF]
, 2012 目的：研究辛二酰苯胺异羟肟酸（suberoylanilidehydroxamicacid，SAHA）对多发性骨髓瘤细胞株U266细胞增殖和凋亡的影响，并探讨其可能机制。 方法：采用台盼蓝拒染法、四氮唑蓝(MTT)比色法检测SAHA对U266细胞增殖的影响；Hoechst33342染色检测凋亡细胞的形态；AnnexinV和PI染色后应用流式细胞仪检测SAHA作用U266细胞的凋亡率；Western-blot方法检测组蛋白H3乙酰化水平 ...
于涛
core
Zhongguo shiyan zhenduanxue, 2009 目的探讨五味子提取物对阿霉素耐药细胞株K562/DOX的耐药逆转作用。方法MTT法检测单独应用阿霉素或阿霉素与五味子提取物联合作用的K562/DOX细胞的生存率;RT-PCR检测MDR1、MRP1 mRNA表达;Westernblotting检测MDR1蛋白的表达。结果与对照组相比,单独应用阿霉素组细胞的生存率未见明显变化;而阿霉素与五味子提取物联合作用组细胞存活率显著降低,MDR1、MRP mRNA表达降低;MDR1蛋白表达下降。结论五味子提取物可能通过下调MDR1、MRP水平进而逆转K562 ...
邱海洋 +6 more
doaj
[Effects of matrine combined with LY294002 on proliferation, apoptosis and cell cycle of human myeloid leukemia K562 cells]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2022 Hao Y, Ji J, Liu C, Zhang N, Gong Y.
europepmc +1 more source
Advances in mitochondrial autophagy regulation of bone remodeling and the role of drug intervention [PDF]
Bone remodeling is a complex process that includes two mutually coordinated phases of bone resorption and bone formation, which plays an important role in preventing bone aging and increasing bone density.
JIANG Honghui +5 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的选择代表不同骨髓细胞分化阶段的血液肿瘤细胞株K-562、HL-60作为研究对象,选取低致病性禽流感病毒H3N2/H4N6作为病原体,探讨禽流感病毒感染骨髓的机制。方法应用流式细胞术检测K562等细胞表面禽流感病毒受体;收集感染病毒的细胞培养上清,红细胞凝集实验检测染毒细胞释放的子代病毒;收集感染病毒的细胞免疫荧光检测病毒NP,判断病毒吸附、进入细胞并在细胞内增殖、复制情况。结果所检测细胞表面均有禽流感病毒SAα2,3-Gal和SAα2,6-Gal连接的唾液酸寡糖受体的表达。在H3N2感染的K ...
张红梅 +4 more
doaj