IL-2,TNFα激活的脐血单核细胞对K562细胞株杀伤作用的研究 附视频
Zhongguo shiyan zhenduanxue, 2003 目的 评价IL - 2 ,TNFα ,IL - 2联合TNFα激活的脐血单核细胞 (Mφ)对白血病细胞株K5 6 2的细胞毒作用。方法 将IL - 2 ,TNFα ,IL - 2联合TNFα分别激活的脐血单核细胞按效靶比 2 0 :1,5 0 :1加入白血病细胞株K5 6 2共同培养 4 8小时 ,采用MTT法检测各组的细胞毒效应。结果 IL - 2 ,TNFα分别激活的脐血单核细胞对白血病细胞株K5 6 2有杀伤作用 ,但较弱 ,而将IL - 2联合TNFα激活的单核细胞对其杀伤作用明显增强 ...
王晓波 +6 more
doaj
α-SEA型地中海贫血标志蛋白zeta链蛋白的克隆表达与鉴定
Zhongguo shiyan zhenduanxue, 2005 目的克隆zeta链蛋白基因并在大肠杆菌中表达,经纯化与鉴定,为建立α-SEA型地中海贫血的免疫学筛查方法提供特异性抗原。方法从经处理的K562细胞中提取总RNA,采用RT-PCR技术反转录并将其克隆到pET-21a（+）载体中,测序验证。融合蛋白在大肠杆菌中表达,采用Probond树脂柱纯化融合蛋白,用WesternBlotting和ELISA鉴定。结果成功构建了融合表达载体pET-zeta,融合蛋白得到可溶性表达及纯化 ...
唐磊 +4 more
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[Expression characteristics and clinical significance of CD109 in de novo acute myeloid leukemia]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2023 Wang LX +9 more
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[Advances in methods and applications of single-cell Hi-C data analysis]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 2023 Gong H, Ma F, Zhang X.
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Zhongshan Daxue xuebao. Yixue kexue ban, 1994 采用体外造血细胞培养技术，应用高温、利多卡因、足叶乙苷（足叶乙甙，ＶＰ－１６）联合法，研究其在体外对白血病细胞的杀伤作用。结果表明：高温、利多卡因、ＶＰ－１６三者联用比高温＋利多卡因、高温＋ＶＰ－１６，能更有效地杀伤白血病细胞，而正常粒单系克隆形成单位（ＧＭ－ＣＦＵ）仍约有５０％存活。４１℃６０ｍｉｎ＋０．７５ｍｍｏｌ／Ｌ利多卡因＋８．５μｍｏｌ／ＬＶＰ－１６联合处理后，ＨＬ－６０、Ｕ９３７、Ｋ５６２细胞分别只存活０．０１％、９．４０％、３３．６６％。另外，从这个联合方案说明对于不同类型的白血病效果不一，
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[Isodopharicin C inhibits NLRP3 inflammasome activation and alleviates septic shock in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2023 Cao H, Zhang W, Li M, Yang Y, Li Y.
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[Preliminary study on the effect of B7H3 interaction with fibronectin on apoptosis of human chronic myeloid leukemia cells]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2021 Sun MY, Xie JJ, Zhang DZ, Zhang GB.
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[Development and functional verification of CAR-T cells targeting CLL-1]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2022 Chai X, Jin X, Zhao MF.
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[Effects of decitabine on proliferation capacity and TFPI-2 expression in leukemia K562 cells]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2017 Wang FJ +5 more
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[Comparison of the characteristics of NK cells after two different methods of expansion and observation of the clinical efficacy in patients who relapsed post allogeneic hematopoietic stem cell transplantation]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2022 Cao XH +13 more
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