Results 51 to 60 of about 545,384 (139)
Proteomic Signatures of Noise‐Induced Hearing Loss in the Mouse Cochlea
ABSTRACT Hearing loss affects over 1.5 billion people worldwide and has substantial social, educational, and economic consequences. Although genetic studies have identified numerous hearing‐loss‐associated genes, the molecular changes accompanying noise‐induced hearing loss (NIHL) remain incompletely understood.
Ana Carla Batissoco +6 more
wiley +1 more source
Kanamycin selection of transformed Arabidopsis
The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium.
Weigel, D. ; https://orcid.org/ +1 more
core +1 more source
Diverse physiological functions for dual-specificity MAP kinase phosphatases [PDF]
A structurally distinct subfamily of ten dual-specificity (Thr/Tyr) protein phosphatases is responsible for the regulated dephosphorylation and inactivation of mitogen-activated protein kinase (MAPK) family members in mammals.
Keyse, Stephen M, Dickinson, Robin J
core +1 more source
Characterization of carABpyrB operon and role of pyrE in Francisella novicida biofilm
Pyrimidine biosynthesis links metabolism to biofilm regulation in Francisella novicida. During nutrient limitation, reduced pyrimidine availability shifts metabolism from uracil salvage to de novo synthesis, increasing biofilm formation. Mutants in carABpyrB exhibit uracil‐dependent growth defects and elevated biofilm:growth ratios, revealing ...
Katie C. Sprinkel +2 more
wiley +1 more source
Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
Zabel, Pim +11 more
core +2 more sources
(A) Western blot analysis of whole cell extracts (left) and supernatant (SN) fractions (right) from P. aeruginosa PAK cells grown under increasing levels of kanamycin (µg/ml) as indicated below the blots. Upper blots are probed with anti-RNA polymlerase (
Jack Horlick (481714) +4 more
core +1 more source
Conserved Asp233–Asp246 hydrogen bond modulates active site dynamics in class A β‐lactamases
Abstract Rigid enzymes catalyze chemical reactions by stabilizing the transition state through a specific conformation. Catalytic residues are precisely positioned, and enzyme dynamics are kept to a minimum. It is thought that conserved residues around the active site (second‐shell residues) play a crucial role in positioning catalytic residues. Asp233
Cristina V. Lopez‐Gallego +3 more
wiley +1 more source
Instability of kanamycin resistance.
The percentages of kanamycin resistant progeny from a number of self-fertilised seed capsules are shown for kr2.3 (A), kr2.5 (B), kr2.7 (C) and kr2.10 (D).
Anna E. Sheppard (375147) +1 more
core +1 more source
Identification of Molecular Pathways Underlying Mn(II) Oxidation in Pseudomonas resinovorans
Transposon mutagenesis in biofilter‐associated Pseudomonas resinovorans MOB‐513 identifies a c‐di‐GMP‐dependent regulatory network involving type IV pili, two‐component systems, and diguanylate cyclases that controls Mn(II) oxidation and biofilm formation, providing new insight into microbial manganese cycling and biofilter performance.
Ainelén Piazza +5 more
wiley +1 more source
We identified a PmWRKY‐10→PmPMT2‐1→PME defence pathway in Pinus massoniana against pine wilt disease. PmWRKY‐10 activates PmPMT2‐1 to promote the accumulation of the nematicidal metabolite PME. This discovery reveals the mechanism of stilbene‐mediated resistance and provides key genetic targets for forest management.
Ziyan Nie +6 more
wiley +1 more source

