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Marker integration and development of Fluidigm/KASP assays for high-throughput genotyping of radish

Horticulture, Environment, and Biotechnology, 2020
Radish (Raphanus sativus L.) is a representative root crop of the Brassicaceae family and is important to the vegetable seed industry in East Asia. Due to its agronomic importance, various molecular markers, genetic maps, genomic resources, and genome assemblies of radish have been developed during the past decade.
Hee-Ju Yu   +5 more
openaire   +1 more source

Genomic Evaluation of Sunflower Broomrape (Orobanche Cumana) Germplasm by KASP Assay

Helia, 2018
AbstractOrobanche cumana Wallr. is a holoparasitic plant for only sunflower, hence it is called as sunflower broomrape. Yield loss created by O. cumana which is generally 50 % can reach to 100 %. In this study, it was planned to perform molecular characterization of O.
Nilay Yonet   +3 more
openaire   +1 more source

SNP Genotyping: The KASP Assay

2014
The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) or inserts and deletions (InDels).
Chunlin, He   +2 more
openaire   +2 more sources

Development and validation of KASP assays for genes underpinning key economic traits in bread wheat

Theoretical and Applied Genetics, 2016
We developed and validated a robust marker toolkit for high-throughput and cost-effective screening of a large number of functional genes in wheat. Functional markers (FMs) are the most valuable markers for crop breeding programs, and high-throughput genotyping for FMs could provide an excellent opportunity to effectively practice marker-assisted ...
Awais Rasheed   +10 more
openaire   +2 more sources

Identification of Novel QTLs/Defense Genes in Spring Wheat Germplasm Panel for Seedling and Adult Plant Resistance to Stem Rust and Their Validation Through KASP Marker Assays

Plant Disease, 2023
Stem rust is one of the major diseases threatening wheat production globally. To identify novel resistance quantitative trait loci (QTLs), we performed 35K Axiom Array SNP genotyping assays on an association mapping panel of 400 germplasm accessions, including Indian landraces, in conjunction with phenotyping for stem rust at seedling and adult plant ...
Anjan Kumar Pradhan   +16 more
openaire   +3 more sources

A high‐throughput KASP assay provides insights into the evolution of multiple resistant mutations in populations of the two‐spotted spider mite Tetranychus urticae across China

Pest Management Science, 2023
AbstractBackgroundThe two‐spotted spider mite (TSSM), Tetranychus urticae (Acari: Tetranychidae), is a cosmopolitan phytophagous pest in agriculture and horticulture. It has developed resistance to many acaricides by target‐site mutations. Understanding the status and evolution of resistant mutations in the field is essential for resistance management.
Xiu‐Jing Shen   +8 more
openaire   +3 more sources

Development and validation of a SNP-based KASP assay for rapid identification of Aphelenchoides besseyi Christie, 1942

Crop Protection, 2020
Abstract Rice white tip nematode, Aphelenchoides besseyi, is a globally important seed-borne pathogen causing serious yield losses to rice. Since A. besseyi is transmitted via seed, its rapid identification is necessary for its management, as well as for breeding, seed certification and quarantine of rice.
Z. Devran, A. Göknur
openaire   +1 more source

Development of molecular markers for the Mi-1 gene in tomato using the KASP genotyping assay

Horticulture, Environment, and Biotechnology, 2016
Tomato (Solanum lycopersicum) is an economically important vegetable worldwide. Root-knot nematodes have a wide host range and cause serious problems in areas where tomatoes are grown. Developing tomato varieties that are resistant to root-knot nematodes is an effective crop management strategy.
Zübeyir Devran   +2 more
openaire   +1 more source

Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion

2019
Marker assisted selection (MAS), an advance tool in plant breeding that allows accurate and efficient introgression of important agronomic trait(s) from a germplasm source to desired elite lines, has been applied to sorghum recently. Here, we report the methods for the deployment of MAS for trait introgression using endpoint genotyping technology for ...
Gloria, Burow   +4 more
openaire   +2 more sources

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