Results 171 to 180 of about 870,917 (183)
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A high‐throughput KASP assay provides insights into the evolution of multiple resistant mutations in populations of the two‐spotted spider mite Tetranychus urticae across China

Pest Management Science, 2023
AbstractBackgroundThe two‐spotted spider mite (TSSM), Tetranychus urticae (Acari: Tetranychidae), is a cosmopolitan phytophagous pest in agriculture and horticulture. It has developed resistance to many acaricides by target‐site mutations. Understanding the status and evolution of resistant mutations in the field is essential for resistance management.
Xiu‐Jing Shen   +8 more
openaire   +3 more sources

Accelerating Public Sector Rice Breeding With High-Density KASP Markers Derived From Whole Genome Sequencing of Indica Rice [PDF]

open access: yesMolecular Breeding, 2018
Few public sector rice breeders have the capacity to use NGS-derived markers in their breeding programmes despite rapidly expanding repositories of rice genome sequence data.
Katherine A Steele
exaly   +2 more sources

SNP discovery from next-generation transcriptome sequencing data and their validation using KASP assay in wheat (Triticum aestivum L.)

Molecular Breeding, 2017
Single nucleotide polymorphisms (SNPs) are becoming the most amenable form of DNA-based molecular markers for genetic analysis. In hexaploid bread wheat (Triticum aestivum L.), it is difficult to discern true polymorphic SNPs due to homoeologous and paralogous genes.
Saket Chandra   +9 more
openaire   +3 more sources

Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp. chinensis Makino

Plant Breeding, 2019
AbstractSingle‐nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non‐heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non‐heading Chinese cabbage inbred lines ...
Peirong Li   +9 more
openaire   +1 more source

Rapid Identification of Meloidogyne Hapla by Kasp Assay

Crop Protection, 2023
Mustafa Çatalkaya   +2 more
openaire   +1 more source

Development and validation of a tetra-primer ARMS-PCR assay for genotyping the MTHFR (rs1801131) c.1286 A > C (p.Glu429Ala) polymorphism: a comparative study with KASP

Molecular Biology Reports
The tetra-primer amplification refractory mutation system (T-ARMS) PCR assay is a cost-effective and rapid method for SNP genotyping. The methylenetetrahydrofolate reductase (MTHFR) c.1286 A > C (rs1801131) polymorphism can influence MTHFR enzyme activity and homocysteine levels.
Amany, Alqosaibi   +6 more
openaire   +2 more sources

Development of High Throughput KASP Assays for Identification of Cytotypes and Fertility Restoration in Onion ( Allium cepa L.)

Plant Breeding
ABSTRACT Cytoplasmic male sterility (CMS) is used for hybrid seed production in onions and S cytoplasm is widely utilized due to its better stability and simple inheritance. In CMS‐S, male fertility is restored by the single dominant allele at the nuclear male fertility restoration locus ( Ms
Priyanka Anand   +5 more
openaire   +1 more source

Prevalence of two acetolactate synthase target site resistance mutations in sunflower broomrape from Europe as determined by robust kompetitive allele‐specific assays ( KASP )

Pest Management Science
Abstract BACKGROUND Orobanche cumana is an important pest of sunflower crops in Europe and Asia. Recently, two highly virulent populations of sunflower broomrape from Greece were found to have evolved resistance to imazamox owing to the A205D and ...
Deepmala Sehgal   +4 more
openaire   +2 more sources

Multiplex fluorescent amplification‐refractory mutation systemPCRmethod for the detection of 10 genetic defects in Holstein cattle and its comparison with theKASPgenotyping assay

Animal Genetics
AbstractThe common deleterious genetic defects in Holstein cattle include haplotypes 1–6 (HH1–HH6), haplotypes for cholesterol deficiency (HCD), bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM) and brachyspina syndrome (BS). Recessive inheritance patterns of these genetic defects permit the carriers to function normally,
Md. Yousuf Ali Khan   +8 more
openaire   +2 more sources

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