Characterization of Haplotype Diversity in the BADH2 Aroma Gene and Development of a KASP SNP Assay for Predicting Aroma in U.S. Rice [PDF]
Rice, 2020 Background Aroma is an important grain quality trait in rice, controlled by mutations within the BADH2 gene. The trait is simply inherited, and its importance in variety development makes it a practical target for marker-assisted selection in applied ...
Christopher K. Addison +6 more
doaj +3 more sources
Molecular sexual determinants in Pistacia genus by KASP assay [PDF]
Molecular Biology Reports, 2021 Abstract I. Background: Pistacia is kind of unisexual plant species that can take 4–5 years to initiate bearing stage of trees in terms of economic value. The fruits of species have an extreme importance in food, health and baking industry as a raw material.
Zeynep Nur, Şahin +3 more
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Development and Exploitation of KASP Assays for Genes Underpinning Drought Tolerance Among Wheat Cultivars From Pakistan [PDF]
Frontiers in Genetics, 2021 High-throughput genotyping for functional markers offers an excellent opportunity to effectively practice marker-assisted selection (MAS) while breeding cultivars. We developed kompetitive allele-specific PCR (KASP) assays for genes conferring drought tolerance in common wheat (Triticum aestivum L.). In total, 11 KASP assays developed in this study and
Shoaib Ur Rehman +10 more
openaire +3 more sources
Ppe.XapF: High Throughput KASP Assays to Identify Fruit Response to Xanthomonas arboricola pv. pruni (Xap) in Peach [PDF]
PLOS ONE, 2021 Abstract Bacterial spot, caused by Xanthomonas arboricola pv. pruni (Xap), is a serious peach disease with symptoms that traverse severe defoliation and black surface pitting, cracking or blemishes on peach fruit with global economic impacts. A management option for control and meeting consumer demand for chemical-free, environmentally friendly
Margaret B. Fleming +5 more
openaire +5 more sources
Validation of KASP assays associated with barley adaptation and productivity traits
Eurasian Journal of Applied Biotechnology, 2022 Barley (Hordeum vulgare L.) is an important cereal crop traditionally used in animal feed, malting, and food production. In Kazakhstan, barley is the second most cultivated cereal grain. However, despite the long history of barley cultivation in Kazakhstan, traditional breeding methods prevail here.
Yu. Genievskaya +4 more
openaire +1 more source
Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons [PDF]
Genes, 2021 Pigeon racing’s recent upturn in popularity can be attributed in part to the huge prize money involved in these competitions. As such, methods to select pigeons with desirable genetic characteristics for racing or for selective breeding have also been gaining more interest.
Ching-Chi Chang +10 more
openaire +2 more sources
Single Nucleotide Polymorphism Analysis Using KASP Assay Reveals Genetic Variability in Rotylenchulus reniformis [PDF]
Plant Disease, 2019 This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using the process of whole genome ...
Churamani Khanal +5 more
openaire +3 more sources
Frontiers in Plant Science, 2021 Optimization of a breeding program for increased genetic gain requires quality assurance (QA) and quality control (QC) at key phases of the breeding process.
Patrick Obia Ongom +8 more
doaj +1 more source
Technical note: Development and application of KASP assays for rapid screening of 8 genetic defects in Holstein cattle [PDF]
Journal of Dairy Science, 2020 Specific DNA mutations underlying several genetic defects associated with embryo loss or reduced calf survivability have been identified in dairy cattle, and a convenient and cost-effective platform is required for their routine screening. We developed Kompetitive allele-specific PCR (KASP) assays for discrimination of the wild-type alleles from the ...
Yi, Zhang +9 more
openaire +2 more sources
Quantitative Trait Loci Mapping and Development of KASP Marker Smut Screening Assay Using High-Density Genetic Map and Bulked Segregant RNA Sequencing in Sugarcane (Saccharum spp.) [PDF]
Frontiers in Plant Science, 2022 Sugarcane is one of the most important industrial crops globally. It is the second largest source of bioethanol, and a major crop for biomass-derived electricity and sugar worldwide. Smut, caused bySporisorium scitamineum, is a major sugarcane disease in many countries, and is managed by smut-resistant varieties.
Yijing Gao +16 more
openaire +3 more sources