Developing Breeder-Friendly KASP Markers for Rust R Genes
Methods in Molecular BiologyThe marker-assisted breeding (MAB) approach has become more convenient and time efficient with the advancement of allele-specific, cost-effective, and breeder-friendly single nucleotide polymorphism (SNP)-based kompetitive allele-specific (KASP) polymerase chain reaction (PCR) markers.
Jyoti Saini Sharma, Lovepreet Singh
exaly +3 more sources
Identification of QTL for Alkylresorcinols in Wheat and Development of KASP Markers for Marker-Assisted Selection of Health-Promoting Varieties [PDF]
This study evaluated alkylresorcinol concentration (ARC) in recombinant inbred lines (RILs) from the cross of Zhongmai 578 and Jimai 22 in three environments. ARC exhibited a continuous distribution ranging from 337.4 to 758.0, 495.4-768.0, and 456.3-764.7 μg/g, respectively, in three environments. Analysis of variance (ANOVA) indicated significant (P <
Lei Zhi +8 more
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Development of KASP Markers in Association with Avirulence Genes and Utilization of a CYP51 KASP Marker to Determine Dynamics of DMI Fungicide Sensitivity in the Stripe Rust Pathogen, Puccinia Striiformis [PDF]
To determine the dynamics of DMI fungicide tolerance in the Pst and Psh populations in the United States, the KASP marker for the point mutation (from nucleotide A to T for the Y134F amino acid change) in the CYP51 gene was used to genotype 3,329 Pst isolates collected from 1968 to 2021 and 583 Psh isolates collected from 1993 to 2021. In addition, 190
Merrill, Hannah
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Accelerating public sector rice breeding with high-density KASP markers derived from whole genome sequencing of indica rice [PDF]
Few public sector rice breeders have the capacity to use NGS-derived markers in their breeding programmes despite rapidly expanding repositories of rice genome sequence data.
Katherine Steele, Steele Katherine A
exaly +3 more sources
Resistance of Capsicum annuum to Phytophthora blight is dependent on the genetic background of the resistance source and the Phytophthora capsici isolate, which poses challenges for development of generally applicable molecular markers for marker-assisted selection. In this study, the resistance to P. capsici of C.
Zhenghai Zhang +10 more
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KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.) [PDF]
Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1.
Dragana Miladinovic +2 more
exaly +3 more sources
Genetic analysis and marker assisted selection using KASP markers in a doubled haploid bread wheat population [PDF]
Bread Wheat breeding can rely on different methods, the main objective of this study was to combine Marker Assisted Selection with haplodiploidisation to accelerate the breeding process, focusing on the introduction of high yield, rust resistance genes ...
Chafika Djenadi +5 more
openaire +2 more sources
Association of KASP markers with Hessian fly resistance in wheat of diverse origin
Euphytica, 2018Hessian fly (Mayetiola destructor (Say)) is a major pest in wheat producing areas of United States, Canada, Europe, and North Africa but has not been found in Australia. Host plant resistance is thought to involve a similar strategy used against plant pathogen, and in this study, we sought to investigate whether disease-resistance gene markers can also
Damian Collins +4 more
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Development of Breeder-Friendly KASP Markers from Genome-Wide Association Studies Results
2022Array-based SNP markers are commonly used in genome-wide association studies (GWAS) to identify genomic regions involved in important agronomical traits. However, conversion of these SNP markers into breeder-friendly kompetitive allele-specific PCR (KASP) markers for use in marker-assisted selection is often challenging.
Manar, Makhoul, Christian, Obermeier
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