Results 21 to 30 of about 22,770 (160)
Construction of new ligation-independent cloning vectors for the expression and purification of recombinant proteins in silkworms using BmNPV bacmid system. [PDF]
PLoS ONE, 2013 A ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-efficiency cloning of genes in a Bombyx mori expression system. This system was confirmed by the expression of human microsomal triglyceride transfer protein (
Tatsuya Kato +2 more
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Escherichia coli alpha-ketoglutarate dehydrogenase complex.
Journal of Biological Chemistry, 1984 The alpha-ketoglutarate dehydrogenase complex from Escherichia coli catalyzes the hydrolysis of S-succinyl-CoA to succinate and CoASH. The reaction rate is dependent upon the presence of thiamin pyrophosphate and NADH, as well as the functional integrity of the alpha-lipoyl groups associated with the enzyme. The Km value for S-succinyl-CoA is 9.3 X 10(-
C A, Steginsky, P A, Frey
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Journal of Cachexia, Sarcopenia and Muscle, 2023 Background This study was designed to develop an understanding of the pathophysiology of traumatic muscle injury in the context of Western diet (WD; high fat and high sugar) and obesity.
Junwon Heo +7 more
doaj +1 more source
Neurobiology of Disease, 2009 The activity of a key mitochondrial tricarboxylic acid cycle enzyme, α-ketoglutarate dehydrogenase complex (KGDHC), declines in many neurodegenerative diseases. KGDHC consists of three subunits.
Lichuan Yang +11 more
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Using Taguchi Method to Determine the Optimum Conditions for Synthesizing Parapyruvate
Molecules, 2022 The synthesis of parapyruvate is important for the analysis of the content in the pyruvate supplements and the study of aging-related neurodegenerative diseases. However, the pure parapyruvate crystal is not, as yet, commercially available. In this study,
Inn Lee, Nae-Cherng Yang
doaj +1 more source
Frontiers in Neurology, 2019 Mitochondria constitute a central role in brain energy metabolism, and play a pivotal role in the development of secondary pathophysiology and subsequent neuronal cell death following traumatic brain injury (TBI).
Jignesh D. Pandya +7 more
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Genetic dissection of the mitochondrial lipoylation pathway in yeast
BMC Biology, 2021 Background Lipoylation of 2-ketoacid dehydrogenases is essential for mitochondrial function in eukaryotes. While the basic principles of the lipoylation processes have been worked out, we still lack a thorough understanding of the details of this ...
Laura P. Pietikäinen +4 more
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Differential Aspartate Usage Identifies a Subset of Cancer Cells Particularly Dependent on OGDH
Cell Reports, 2016 Although aberrant metabolism in tumors has been well described, the identification of cancer subsets with particular metabolic vulnerabilities has remained challenging.
Eric L. Allen +17 more
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Antioxidants, 2021 Since breast cancer (BC) cells are dependent on mitochondrial bioenergetics for promoting proliferation, survival, and metastasis, mitochondria highlight as an important target for anticancer drug discovery.
Miguel Córdova-Delgado +7 more
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Mechanistic effects of amino acids and glucose in a novel glutaric aciduria type 1 cell model. [PDF]
PLoS ONE, 2014 Acute neurological crises involving striatal degeneration induced by a deficiency of glutaryl-CoA dehydrogenase (GCDH) and the accumulation of glutaric (GA) and 3-hydroxyglutaric acid (3-OHGA) are considered to be the most striking features of glutaric ...
Xi Fu +7 more
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