Results 71 to 80 of about 369,315 (311)

Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase [PDF]

, 2011
A previously determined crystal structure of the ternary complex of trehalose-6-phosphate synthase identified a putative transition state–like arrangement based on validoxylamine A 6?-O-phosphate and uridine diphosphate in the active site.
Errey, James C., Gideon J Davies, Hong, SY, Izumi, A, Davis, BG, James C Errey, Benjamin G Davis, Hong, Sung You, Izumi, Atsushi, Atsushi Izumi, Errey, JC, Lee, Seung Seo, Sung You Hong, Lee, SS, Davies, Gideon J., Seung Seo Lee, Davis, Benjamin G., Davies, GJ, Davis, Benjamin   +18 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Enlarged Lumen Volume of Proximal Aortic Segment and Acute Type B Aortic Dissection: A Computer Fluid Dynamics Study of Ideal Aortic Models

International Journal of General Medicine, 2022
Yuan Peng,1 Xuelan Zhang,2 Jiehua Li,1 Xiaolong Zhang,1 Hao He,1 Xin Li,1 Kun Fang,3 Liancun Zheng,2 Chang Shu1,3 1Department of Vascular Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, People’s Republic of China; 2School of
Peng Y, Zhang X, Li J, Zhang X, He H, Li X, Fang K, Zheng L, Shu C   +8 more
doaj  

PLAYING WITH A CHAIN OR PHYSICAL AND MATHEMATICAL INFORMATICS

Современные информационные технологии и IT-образование, 2018
The article describes an educational laboratory work within the framework of interdisciplinary connec- tions at the intersection of informatics, mathematics and physics: the study of the sagging of a closed chain with different support points.
Valery F. Ochkov, Massimiliano Nori
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Physlets Tour 5: Conversion from Kinetic Energy to Thermal Energy

, 2001
A heavy ball with an initial kinetic energy of 4000 J is trapped inside a box with rigid walls containing a cylinder constructed of small lightweight spheres. The vertical bar on to the right of each frame shows the kinetic energy of the ball.
Christian, Wolfgang; Belloni, Mario
core   +1 more source

Thermogravimetric and kinetic analysis of energy crop Jerusalem artichoke using the distributed activation energy model [PDF]

, 2013
Jerusalem artichoke has great potential as future feedstock for bioenergy production because of its high tuber yield (up to 90 t ha(-1)), appropriate biomass characteristics, low input demand, and positive environmental impact.
Qin, Song, Wang, Shaoyu, Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. sq0535@163.com, Li, Lili, Wang, Gang   +4 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Harvesting of Kinetic Energy of the Raindrops

, 2014
This paper presents a methodology to harvest the kinetic energy of the raindrops using piezoelectric devices. In the study 1m×1m PVDF (Polyvinylidene fluoride) piezoelectric membrane, which is fixed by the four edges, is considered for the numerical simulation on deformation of the membrane due to the impact of the raindrops. Then according to the drop
K. C. R. Perera, B. G. Sampath, V. P. C. Dassanayake, B. M. Hapuwatte.   +3 more
openaire   +1 more source