Results 81 to 90 of about 3,799,045 (306)

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

Kinetic Measurement of the Erythrocyte Sedimentation Rate

open access: yesUpsala Journal of Medical Sciences, 1994
A method for estimation of the erythrocyte sedimentation from repeated measurements during the initial part of the sedimentation phase is described. Based on the measurements between 18 and 24 minutes after the beginning of the reaction, the sedimentation at 60 minutes is estimated.
A, Kallner, P, Engervall, M, Björkholm
openaire   +2 more sources

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

open access: yesFEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan   +2 more
wiley   +1 more source

Chemical Characterization and Kinetic parameter determination under Rancimat test conditions of four monovarietal virgin olive oils grown in Morocco

open access: yesOilseeds and fats, crops and lipids, 2016
The aim of the present investigation is to compare the chemical characterization of four monovarietal virgin olive oils obtained from fruits of olive trees grown in Morocco (Picholine, Picual, Arebiquine, Koroneiki) with kinetic ...
Gharby Said   +5 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

open access: yesFEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

13C labeling experiments at metabolic nonstationary conditions: An exploratory study

open access: yesBMC Bioinformatics, 2008
Background Stimulus Response Experiments to unravel the regulatory properties of metabolic networks are becoming more and more popular. However, their ability to determine enzyme kinetic parameters has proven to be limited with the presently available ...
Wiechert Wolfgang   +2 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

open access: yesFEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata   +3 more
wiley   +1 more source

Experimental verification of effects of turbulent boundary layers on chemical-kinetic measurements in a shock tube [PDF]

open access: yes, 1970
Turbulent boundary layer effects on chemical and kinetic measurements of gas mixture composition from nonuniform shock tube ...
Belles, F. E., Brabbs, T. A.
core  

Steady state kinetic parameters.

open access: yes, 2015
Enzymatic activities are measured at various concentrations of GSH and PITC as described in the Experimental section. Kinetic constants are based on three independent experiments for each measurement.
Gaurav Chhetri (737902)   +4 more
core   +1 more source

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