Results 121 to 130 of about 411,207 (359)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Decoupling fast hydrogen oxidation reaction on a tandem electrocatalyst

Nature Communications
The hydrogen oxidation reaction (HOR) shows fast kinetics in proton exchange membrane fuel cells (PEMFCs), and has not drawn intense attention. Here, we propose a tandem electrocatalysis concept, decoupling HOR on two independent active sites for ...
Wei Guo, Guoqiang Zhao, Ziang Sun, Bingxing Zhang, Dongyue Xin, Mingxia Gao, Yongfeng Liu, Zhongbin Zhuang, Hai-Wei Liang, Hongge Pan, Wenping Sun   +10 more
doaj   +1 more source

Single-molecule interaction microscopy reveals antibody binding kinetics [PDF]

, 2020
Thilini Perera, Hirushi Gunasekara, Ying Hu   +2 more
openalex   +1 more source

Structure-function study of mammalian Munc18-1 and C. elegans UNC-18 implicates domain 3b in the regulation of exocytosis

, 2011
This work was supported by grants from the Wellcome Trust and BBSRC. GRP was supported by a Wellcome Trust Prize Studentship. The C. elegans strain used in this work was provided by the Caenorhabditis Genetics Center, which is funded by the National ...
Haynes, Lee P., Burgoyne, Robert D., Walmesley Alice, Burgoyne Robert D., James R Johnson, Gerald R. Prescott, Barclay, Jeff W., Robert D. Burgoyne, Johnson James R., James R. Johnson, Prescott, Gerald R., Gerald R Prescott, Lee P. Haynes, Morgan, Alan, Margaret E Graham, Walmesley, Alice, Johnson, James R., Jeff W Barclay, Prescott Gerald R., Jeff W. Barclay, Lee P Haynes, Jones Mathew, Graham, Margaret E., Morgan Alan, Margaret E. Graham, Jones, Mathew, Alan Morgan, Barclay Jeff W., Robert D Burgoyne, Mathew Jones, Graham Margaret E., Haynes Lee P., Alice Walmesley   +32 more
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Mechanism and kinetics of uric acid adsorption on nanosized hydroxyapatite coating

Journal of Saudi Chemical Society, 2019
Uric acid (UA) produced from purine metabolism is rather harmful to human health when its concentration is high. To better understand the application of hydroxyapatite (HAP) as an adsorbent for UA removal, quartz crystal microbalance (QCM) technique was ...
Chunlei Zhang, Zhiguo Xiao, Tongtong Qin, Zhengpeng Yang   +3 more
doaj   +1 more source

Release kinetics and binding interactions of curcumin‐encapsulated nanoparticles released from chitosan‐graft‐(methoxy poly(ethylene glycol)‐block‐polycaprolactone) injectable hydrogels [PDF]

, 2023
Pawitchaya Madech, Napasri Sitthichai, Chanita Phetdee, Panchika Prangkio, Winita Punyodom, Kiattikhun Manokruang   +5 more
openalex   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Growth kinetics and power laws indicate distinct mechanisms of cell-cell interactions in the aggregation process

, 2021
Debangana Mukhopadhyay, Rumi De
openalex   +2 more sources

Kinetics of Auxin Interaction [PDF]

Plant Physiology, 1953
D H, McRae, R J, Foster, J, Bonner
openaire   +2 more sources