Results 111 to 120 of about 173,856,276 (259)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Determine interaction affinity changes of the SUMO E1 activating enzymes during SUMO activation using qFRET technology

Medicine in Drug Discovery
The heterodimeric E1 complex, Aos1-Uba2, catalyzes the first adenylation activation of the SUMO1 peptide in the SUMOylation cascade. The reaction affinity and dynamics of the Aos1-Uba2 heterodimer during the first step activation have yet to be ...
Ling Jiang, Yiran Tao, Xin Wen, Jiayu Liao   +3 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Modeling Fischer Tropsch synthesis in two-phase, continuous, well-mixed slurry reactors [PDF]

, 2009
Includes abstract.Includes bibliographical references (p. 84-87).Fischer Tropsch Synthesis (FTS) is the conversion of syngas (CO and H2) to cleaner liquid transportation fuels.
Mthombeni, Bongani
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Binding kinetics and affinities for interaction of Mtb deacylated substrates with LprG and LprG-V91W.

, 2014
Values were calculated using BIAevaluation 3.1 software. Data shown are representative of at least three independent experiments.Binding kinetics and affinities for interaction of Mtb deacylated substrates with LprG and LprG-V91W.
Supriya Shukla (651747), Jaffre J. Athman (651749), David McDonald (95388), Niaz Banaei (123779), Libin Shi (651750), W. Henry Boom (125099), Clifford V. Harding (334250), Edward T. Richardson (651748), Pamela A. Wearsch (651751), Mary Jackson (73333)   +9 more
core   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Kinetics and mechanism of the reaction of acetonyl radical, CH3(CO)CH2, with Br2 [PDF]

, 2013
The low pressure fast discharge flow method with laser induced fluorescence detection of CH3C(O)CH2 was employed to study the kinetics of the reaction CH3C(O)CH2 + Br2 → CH3C(O)CH2Br + Br (1) at 298, 323 and 365 K.
Zügner, Gábor L., Brudnik, Katarzyna, Szabó, Emese, Farkas, Mária, Jodkowski, Jerzy T., Dóbé, Sándor, Sarzynski, Dariusz   +6 more
core  

Rheokinetics of Cement Paste Hydration during the Dormant Phase

Applied Rheology, 2018
The hydration kinetics of four CEM I 52.5 N cements in the presence of two different superplasticizers was studied. The kinetics was characterized by monitoring the evolution of the dynamic modulus at constant frequency on a rotational rheometer.
Masalova Irina, Mbasha Willy, Haldenwang Rainer, Malkin Alexander Ya   +3 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source