Results 61 to 70 of about 107,528 (262)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
The effect of knocking down Sec31A on the malignant phenotype of HNSCC [PDF]
Kouqiang yixueObjective To explore the impact of knocking down Sec31A on the malignant phenotype of head and neck squamous cell carcinoma(HNSCC) and its possible mechanisms. Methods Transcriptome sequencing data of HNSCC tissues and adjacent tissues were obtained from
HE Yao, ZHAO Zhenyuan, GAO Teng, LIN Peng, CHEN Yiren, SONG Xiaomeng
doaj +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
A Knock Detection Method for a Marine Micro-Ignition Dual-Fuel Engine Based on Vibration Signals
Polish Maritime ResearchA simple and unified index is proposed to achieve knock detection under various engine loads. Maximum amplitude vibration oscillation (MAVO) and maximum amplitude pressure oscillation (MAPO) were compared and were found to have no consistency. This means
Hu Nao +7 more
doaj +1 more source
KNOCK, KNOCK, KNOCK-IN ON GABA’S DOOR: GABRB3 Knock-in Mutation Causes Infantile Spasms in Mice
Epilepsy Currents, 2023 [Box: see text]
openaire +2 more sources
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
FEBS Open Bio, EarlyView.Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source
Research of Analysis and Diagnosis of Abnormal Noise at Idle Speed of a Truck Transmission
Jixie chuandong, 2021 Aiming at the problem of transmission gear idling knocking abnormal noise in a batch of domestically produced new trucks， based on the research method of "conjecture-theoretical research-test verification"， the situation that the transmission idling ...
Liangliang Zhao +4 more
doaj
Wild forest elephants shake down fruit and leaves from trees
Pachyderm, 2002 This short paper reviews aspects of observed feeding behaviour of forest elephants in the vicinity Bomassa near of the headquarters of Nouabale-Ndoki NP, near the Dzanga Clearing and in Gamba.
Fiona Maisels +2 more
doaj +1 more source